AB217622

Anti-CENPA antibody

Name: Anti-CENPA antibody
Brand: Abcam Limited
SKU: AB217622
Rating: 1 (1 reviews)

(1 리뷰)

(4 제품이 사용된 논문 )

Rabbit Polyclonal CENPA antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CENPA aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

Histone H3-like centromeric protein A, Centromere autoantigen A, Centromere protein A, CENP-A, CENPA

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody (AB217622)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody (AB217622)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human gastric carcinoma tissue labeling CENPA with ab217622 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CENPA aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P49450

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CENPA also known as Centromere Protein A is an essential component of the centromere specifically during cell division. It represents a histone H3 variant with an approximate mass of 17 kDa. This protein is expressed at the centromere regions of chromosomes where it substitutes conventional histone H3. CENPA facilitates the assembly of the kinetochore an important structure for chromosome segregation. Scientists also refer to this protein as CENP-A often in conjunction with studies involving anti-centromere antibodies and centromere staining.

Biological function summary

CENPA plays a central role in accurate chromosome segregation during mitosis and meiosis. It incorporates into nucleosomes at the centromere replacing standard histone H3 to promote kinetochore formation. CENPA is part of a larger protein complex known as the centromere. This complex propelled by CENPA drives proper attachment of chromosomes to spindle microtubules preventing aneuploidy.

Pathways

The kinetochore pathway and the chromosome segregation pathway both involve CENPA significantly. It functions in tandem with other centromeric proteins such as CENP-B and CENP-C which work collaboratively to sustain centromere integrity. CENPA interacts with these proteins to orchestrate correct alignment and separation of chromosomes integrating it into the foundational architecture of genetic stability.

CENPA has association with several chromosomal instability syndromes. Misregulation or mutation of this protein may contribute to cancer progression where improper chromosome segregation is an important factor. It also connects to other proteins like CENP-H implicated in malignancies due to errors in chromosome transmission. Additionally autoimmune diseases often involve centromere antibodies highlighting the connection of CENPA to disorders such as systemic sclerosis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed : 11756469, PubMed : 14667408, PubMed : 15282608, PubMed : 15475964, PubMed : 15702419, PubMed : 17651496, PubMed : 19114591, PubMed : 20739937, PubMed : 27499292, PubMed : 7962047, PubMed : 9024683). Replaces conventional H3 in the nucleosome core of centromeric chromatin that serves as an assembly site for the inner kinetochore (PubMed : 18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed : 26878239, PubMed : 27499292). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed : 15282608, PubMed : 15475964, PubMed : 20739937, PubMed : 21478274, PubMed : 26878239). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed : 11756469, PubMed : 14667408, PubMed : 18072184, PubMed : 23818633, PubMed : 25556658, PubMed : 27499292).

See full target information CENPA

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 15:26672 PubMed40696131

2025

The P4HB/LGALS3BP/NF-kB axis promotes the progression of MIA to IAC in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Mu,Wenhao Zhang,Yan Gu,Hongchang Wang,Jingjing Huang,Chengyu Bian,Ke Wei,Yang Xia,Jun Wang

Biomedicines 12: PubMed39200236

2024

The Impact of DAXX, HJURP and CENPA Expression in Uveal Melanoma Carcinogenesis and Associations with Clinicopathological Parameters.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandros Pergaris,Georgia Levidou,Georgios Mandrakis,Maria-Ioanna Christodoulou,Michail V Karamouzis,Jerzy Klijanienko,Stamatios Theocharis

International journal of molecular sciences 23: PubMed35955489

2022

Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Georgia Levidou,Konstantinos Palamaris,Alexandros G Sykaras,Georgios Andreadakis,Christos Masaoutis,Irene Theochari,Penelope Korkolopoulou,Dimitra Rontogianni,Stamatios Theocharis

Digestive surgery 39:24-31 PubMed34788753

2021

Identification and Validation of Hub Genes Predicting Prognosis of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyan Chen,Haitao Yu,Lijun Wu,Sina Zhang,Zhihui Lin,Tuo Deng,Bangjie He,Zhengping Yu,Gang Chen,Fang Wu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CENPA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody (AB217622)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise