Anti-Ceramide glucosyltransferase antibody

• WB

Lab

Western blot - Anti-Ceramide glucosyltransferase antibody (AB124296)

Western blot : Anti-Ceramide glucosyltransferase antibody ab124296 staining at 1 µg/mL, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 50 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/mL

Lane 1:

A431 at 20 µg

Lane 2:

MCF7 at 20 µg

Lane 3:

Human Brain at 20 µg

Lane 4:

Mouse Spleen at 20 µg

Lane 5:

Mouse Brain at 20 µg

Lane 6:

THP-1 at 20 µg

Lane 7:

HL-60 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 45 kDa

Observed band size: 50 kDa

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• WB

Unknown

Western blot - Anti-Ceramide glucosyltransferase antibody (AB124296)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124296 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/ko/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Lane 4:

Spleen (Mouse) Tissue Lysate at 10 µg

Lane 5:

Spleen (Rat) Tissue Lysate at 10 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

A375 (Human melanoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 45 kDa

Observed band size: 12 kDa,36 kDa,48 kDa,68 kDa

true

Exposure time: 30s