Anti-Ceruloplasmin antibody [EPSISR6-12] - BSA and Azide free

• WB

Lab

Western blot - Anti-Ceruloplasmin antibody [EPSISR6-12] - BSA and Azide free (AB232055)

This data was developed using ab131220, the same antibody clone in a different buffer formulation.

Western blot : Anti-Ceruloplasmin antibody [EPSISR6-12] ab131220 staining at 1/1000 dilution, shown in black; Rabbit anti-ACTN4 ab108198 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 122 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit HRP (H+L) and at 1/20,000 dilution.

All lanes:

Western blot - Anti-Ceruloplasmin antibody [EPSISR6-12] (<a href='/ko/products/primary-antibodies/ceruloplasmin-antibody-epsisr6-12-ab131220'>ab131220</a>) at 1/1000 dilution

Lane 1:

OVCAR-3, treated with brefeldin A (5 µg/mL, 6 h) at 20 µg

Lane 2:

OVCAR-3, vehicle control for brefeldin A (0 µg/mL, 6 h) at 20 µg

Secondary

All lanes:

Goat anti-Rabbit HRP (H+L) & at 1/20000 dilution

Predicted band size: 122 kDa

Observed band size: 122 kDa

true

Exposure time: 120s

• WB

Supplier Data

Western blot - Anti-Ceruloplasmin antibody [EPSISR6-12] - BSA and Azide free (AB232055)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131220).

All lanes:

Western blot - Anti-Ceruloplasmin antibody [EPSISR6-12] - BSA and Azide free (ab232055) at 1/1000 dilution

Lane 1:

Human plasma at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Rat liver lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 122 kDa

false