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AB184957

Anti-CES2 antibody [EPR14856]

1

(1 리뷰)

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(4 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal EST2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications.

대체 명칭 보기

ICE, CES2, Cocaine esterase, Carboxylesterase 2, Methylumbelliferyl-acetate deacetylase 2, CE-2, hCE-2

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody [EPR14856] (AB184957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody [EPR14856] (AB184957)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CES2 with ab184957 at 1/500 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-CES2 antibody [EPR14856] (AB184957)
  • WB

Supplier Data

Western blot - Anti-CES2 antibody [EPR14856] (AB184957)

All lanes:

Western blot - Anti-CES2 antibody [EPR14856] (ab184957) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

false

Western blot - Anti-CES2 antibody [EPR14856] (AB184957)
  • WB

Supplier Data

Western blot - Anti-CES2 antibody [EPR14856] (AB184957)

All lanes:

Western blot - Anti-CES2 antibody [EPR14856] (ab184957) at 1/1000 dilution

All lanes:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-CES2 antibody [EPR14856] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14856

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB137606

Anti-CES2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody (AB137606)

  • 0
  • 0
Primary Antibodies

AB126970

Anti-CES2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody (AB126970)

  • 0
  • 0
Primary Antibodies

AB88439

Anti-CES2 antibody

Western blot - Anti-CES2 antibody (AB88439)

  • 0
  • 0
Primary Antibodies

AB215042

Anti-CES2 antibody

Western blot - Anti-CES2 antibody (AB215042)

  • 0
  • 0

보완 제품

Assay Kits

AB313529

Human CES2 ELISA Kit

Sandwich ELISA - Human CES2 ELISA Kit (AB313529)

  • 0
  • 0
Primary Antibodies

AB45957

Anti-Liver Carboxylesterase 1/CES1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Carboxylesterase 1/CES1 antibody (AB45957)

  • 0
  • 0
Primary Antibodies

AB129165

Anti-IMPDH2 antibody [EPR8365(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-IMPDH2 antibody [EPR8365(B)] (AB129165)

  • 0
  • 0
Primary Antibodies

AB181603

Anti-GAPDH antibody [EPR16884] - Loading Control

20ul selling size|RabMAb|Recombinant|Lab Essentials

Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR16884] - Loading Control (AB181603)

  • 5
  • 6
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Carboxylesterase 2 (CES2) also known as intestinal carboxylesterase is an enzyme with a molecular mass of approximately 60 kDa. CES2 hydrolyzes esters and amides affecting metabolism of drugs and xenobiotics. It is found mainly in the liver and small intestine with lesser expression in the colon kidney and brain. This distribution enables CES2 to play a role in both intestinal and systemic drug metabolism.
Biological function summary

CES2 acts in the hydrolysis process converting prodrugs into active compounds. It functions independently and is not typically part of a larger complex. This enzyme demonstrates broad substrate specificity impacting the metabolic activation of several therapeutic agents. Its activity affects the bioavailability and activation of medicines such as irinotecan and capecitabine which are used in cancer treatments.

Pathways

CES2 takes part in the drug metabolism pathway involving cytochrome P450 enzymes. It interacts with CYP3A4 one of the key enzymes in this system and facilitates the conversion of chemical compounds into pharmacologically active or inactive metabolites. CES2 also participates in lipid metabolism affecting the ester and triglyceride hydrolysis alongside lipases and other related enzymes.

CES2 has implications in colorectal cancer and liver disease conditions where altered drug metabolism influences disease progression and treatment efficacy. It shows associations with irinotecan activation a drug used for treating colorectal cancer and links with UDP-glucuronosyltransferases (UGTs) that also engage in drug metabolism. Understanding CES2's function aids in predicting patient responses to drug therapies and refining treatment strategies for these conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed : 9169443). Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine (PubMed : 9169443). Hydrolyzes aspirin, substrates with large alcohol group and small acyl group and endogenous lipids such as triacylglycerol (PubMed : 28677105). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed : 21049984).
See full target information CES2
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta pharmacologica Sinica 46:777-793 PubMed39496863

2024

Intestinal human carboxylesterase 2 (CES2) expression rescues drug metabolism and most metabolic syndrome phenotypes in global Ces2 cluster knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Geng Wang,Chang-Pei Gan,Joke Beukers-Korver,Hilde Rosing,Wen-Long Li,Els Wagenaar,Maria C Lebre,Ji-Ying Song,Colin Pritchard,Rahmen Bin Ali,Ivo Huijbers,Jos H Beijnen,Alfred H Schinkel

Cell regeneration (London, England) 11:19 PubMed35511361

2022

Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Haonan Li,Xiaodan Wang,Yalong Wang,Mengxian Zhang,Fan Hong,Hong Wang,Along Cui,Jianguo Zhao,Weizhi Ji,Ye-Guang Chen

Cells 11: PubMed35053380

2022

Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyao Qin,Yu Xin,Yong Bian,Xuan Yang,Tao Xi,Jing Xiong

Oncotarget 7:75279-75292 PubMed27661117

2016

High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers.

Applications

WB, IHC-P

Species

Human, Human

Jiekai Yu,Xiaofen Li,Chenhan Zhong,Dan Li,Xiaohui Zhai,Wangxiong Hu,Cheng Guo,Ying Yuan,Shu Zheng
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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