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AB92738

Anti-CGA/hCG alpha antibody [EP3373]

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal GLHA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.
2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (AB92738)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (AB92738)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CGA/hCG alpha with ab92738 at 1/600 dilution (0.87 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Western blot - Anti-CGA/hCG alpha antibody [EP3373] (AB92738)
  • WB

Lab

Western blot - Anti-CGA/hCG alpha antibody [EP3373] (AB92738)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1 - 2:

Western blot - Anti-CGA/hCG alpha antibody [EP3373] (ab92738) at 1/2000 dilution

Lane 1:

Western blot - Anti-PKR antibody [EPR19374] (<a href='/ko/products/primary-antibodies/pkr-antibody-epr19374-ab184257'>ab184257</a>) at 1/1000 dilution

Lane 1:

JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate

Lane 1:

bEnd.3 (mouse brain endothelial cell) whole cell lysate at 20 µg

Lane 2:

JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate treated with Hydroxyurea (10mM 24h)

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa

false

Exposure time: 180s

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-CGA/hCG alpha antibody [EP3373] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3373

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB100533

Human hCG ELISA Kit

Sandwich ELISA - Human hCG ELISA Kit (AB100533)

  • 0
  • 0
Primary Antibodies

AB131170

Anti-hCG beta antibody [EPHCGR2]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [EPHCGR2] (AB131170)

  • 0
  • 0
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB82157

Anti-liver FABP antibody [2G4]

Western blot - Anti-liver FABP antibody [2G4] (AB82157)

  • 0
  • 0
Primary Antibodies

AB191077

Anti-Nkx2.2 antibody [EPR14638] - N-terminal

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (AB191077)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": "<p><strong>For unpurified, use 1/100 - 1/250.</strong></p>" } } }
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제품 세부 정보

This product has switched from a hybridoma to recombinant production method on 2nd August 2023.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CGA also known as the hCG alpha subunit is a glycoprotein hormone free alpha subunit. Its molecular mass is approximately 15-22 kDa. It is a part of the human chorionic gonadotropin (hCG) protein. It is expressed in the placenta and various neoplastic tissues. CGA along with the beta subunit forms the complete structure of hCG which plays a significant role in signaling for the reproductive processes.
Biological function summary

This alpha subunit plays a fundamental role in forming the heterodimer with different beta subunits of hCG TSH LH and FSH resulting in various glycoprotein hormones. These hormones are essential for maintaining pregnancy fetal development and regulating the menstrual cycle. The CGA protein is key to the activity of the hCG protein which significantly impacts fetal and trophoblastic growth during pregnancy. The heterodimeric complex to which it contributes assigns it unique biological roles that differ depending on the linked beta subunit.

Pathways

The CGA subunit functions within the reproductive hormone signaling pathway. The uniform alpha subunit pairs with distinct beta subunits such as LH beta or FSH beta influencing different signaling pathways. It plays a vital part in the hormonal regulation pathway modulating endocrine and reproductive processes. The related proteins include the beta subunits like hCG beta LH beta and FSH beta which pair with CGA to perform distinct biological functions in different tissues.

CGA's expression relates to trophoblastic diseases and certain types of cancer such as choriocarcinoma. Aberrant expression or regulation can be seen in these conditions linking it to tumorigenesis and abnormal reproductive disorders. Choriocarcinoma significantly involves hCG protein and its subunits where CGA influences the progression by contributing to this hormone's abnormal activity. Elevated levels of CGA can correlate with the progression of these diseases serving as a clinical marker for diagnosis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH, follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.
See full target information CGA

대체 명칭 보기

Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, Luteinizing hormone alpha chain, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, CG-alpha, FSH-alpha, LSH-alpha, TSH-alpha, CGA
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Annals of medicine and surgery (2012) 86:2644-2650 PubMed38694326

2024

S100A6 could not promote the differentiation of Calu-6 lung cancer cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Ting Wang,Dan Dang

Pituitary 18:31-41 PubMed24402129

2014

Expression of the pituitary stem/progenitor marker GFRα2 in human pituitary adenomas and normal pituitary.

Applications

Unspecified application

Species

Unspecified reactive species

Nestoras Mathioudakis,Ram Sundaresh,Alexandra Larsen,William Ruff,Jennifer Schiller,Hugo Guerrero-Cázares,Peter Burger,Roberto Salvatori,Alfredo Quiñones-Hinojosa
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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