AB236981

Anti-ch TOG antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal ch TOG antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CKAP5 aa 1800 to C-terminus.

대체 명칭 보기

KIAA0097, CKAP5, Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, Ch-TOG

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ch TOG antibody (AB236981)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ch TOG antibody (AB236981)

Paraffin-embedded human kidney tissue stained for ch TOG with ab236981 at a 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ch TOG antibody (AB236981)

Paraffin-embedded human brain tissue stained for ch TOG with ab236981 at a 1/400 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ch TOG antibody (AB236981)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ch TOG antibody (AB236981)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for ch TOG (Green) using ab236981 at a 1/133 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L).

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CKAP5 aa 1800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14008

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ch-TOG also known as colonic and hepatic tumor overexpressed gene serves as an important player in microtubule dynamics. With a molecular weight of approximately 217 kDa this protein is an important component in microtubule polymerization. It is expressed in various tissues including the brain and testis demonstrating its diverse role in cellular structure and division processes. As an integral part of the microtubule organizing center ch-TOG directly contributes to the assembly and stability of the mitotic spindle.

Biological function summary

Ch-TOG is highly involved in cell cycle regulation and mitosis. By interacting with microtubules it maintains the proper formation of the mitotic spindle ensuring accurate chromosome segregation in eukaryotic cells. It functions within larger protein complexes such as the Augmin complex which assists in spindle microtubule nucleation. This protein's ability to promote microtubule growth is essential for dynamic cellular events particularly during cell division.

Pathways

Ch-TOG plays a significant role in the cell cycle and mitotic spindle assembly pathways. It closely associates with proteins such as TACC3 and Aurora A kinase which regulate mitotic spindle formation and stability. In the TACC3-Aurora A pathway ch-TOG helps enhance microtubule assembly a process necessary for successful cell proliferation and division. The interaction with these critical proteins illustrates its contribution to maintaining cellular integrity during mitosis.

Ch-TOG links to cancer progression and developmental abnormalities. Its overexpression often appears in various types of cancer such as colorectal and liver cancers due to its role in promoting uncontrolled cell division. In these circumstances ch-TOG interacts with oncogenic pathways potentially involving proteins like p53 to facilitate tumor growth. Additionally mutations or dysregulation in ch-TOG can lead to improper mitotic progression contributing to developmental disorders related to cell division anomalies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as a processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed : 23532825). Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments (PubMed : 27156448).

See full target information CKAP5

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

eLife 13: PubMed40047807

2025

Impaired yolk sac NAD metabolism disrupts murine embryogenesis with relevance to human birth defects.

Applications

Unspecified application

Species

Unspecified reactive species

Kayleigh Bozon,Hartmut Cuny,Delicia Z Sheng,Ella M M A Martin,Alena Sipka,Paul Young,David T Humphreys,Sally L Dunwoodie

Cell reports 31:107681 PubMed32460023

2020

Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Stiff,Fabio R Echegaray-Iturra,Harry J Pink,Alex Herbert,Constantino Carlos Reyes-Aldasoro,Helfrid Hochegger

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ch TOG antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ch TOG antibody (AB236981)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ch TOG antibody (AB236981)

Immunocytochemistry/ Immunofluorescence - Anti-ch TOG antibody (AB236981)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise