AB243505

Anti-CHCH antibody

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Rabbit Polyclonal CHCH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CHURC1 aa 1-150.

대체 명칭 보기

C14orf52, CHCH, My015, CHURC1, Protein Churchill

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHCH antibody (AB243505)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHCH antibody (AB243505)

Formalin-fixed, paraffin-embedded human colon tissue stained for CHCH using ab243505 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CHCH antibody (AB243505)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CHCH antibody (AB243505)

PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma derived from bone) cells stained for CHCH (green) using ab243505 at 4 μg/ml in ICC/IF analysis.

Supplier Data

Western blot - Anti-CHCH antibody (AB243505)

All lanes:

Western blot - Anti-CHCH antibody (ab243505) at 0.4 µg/mL

Lane 1:

Empty vector transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

HEK-293T overexpressing CHCH with C-terminal myc-DDk tag (~3.1 kDa).

Predicted band size: 13 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CHURC1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q8WUH1

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The CHCH domain-containing protein also known as CHCHD plays a mechanical role within the cell by being involved in mitochondrial processes. It has a mass of approximately 15 kDa. This protein is expressed in various tissues with high levels in the heart and skeletal muscles. It localizes to the mitochondria where it interacts with other mitochondrial proteins to maintain cellular function.

Biological function summary

The CHCHD protein participates in the assembly and stabilization of protein complexes within the mitochondria. It serves as a component of the mitochondrial intermembrane space bridging complex (MIB) facilitating electron transfer processes necessary for cellular respiration. The stability of the complex is essential for maintaining the proper mitochondrial bioenergetics that influence overall cellular health.

Pathways

The CHCHD protein is an important player in mitochondrial respiration and apoptosis pathways. It interacts with cytochrome c during the electron transport chain which is important for ATP production. This protein also relates to apoptosis through interactions that influence the release of pro-apoptotic factors like c-caspases supporting the balance between cell survival and programmed cell death.

The CHCHD protein associates with neurodegenerative diseases such as Parkinson's disease where its dysfunction contributes to mitochondrial abnormalities. It also connects with cardiac disorders since it influences the mitochondrial pathways important for heart function. In Parkinson's disease alterations in interactions between CHCHD and proteins like CHCHD2 have been implicated highlighting the protein's role in mitochondrial health and disease.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcriptional activator that mediates FGF signaling during neural development (By similarity). Plays a role in the regulation of cell movement (By similarity).. Isoform 4. Does not bind DNA by itself.

See full target information CHURC1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Tohoku journal of experimental medicine : PubMed41062300

2025

Identification of KLHL35, WDR72, and WDR78 as Prognostic Biomarkers in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayou Ye,Tingting Zhang,Guangsheng Wang,Guofeng Bian,Aijun Chen,Xin Zhou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CHCH antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHCH antibody (AB243505)

Immunocytochemistry/ Immunofluorescence - Anti-CHCH antibody (AB243505)

Western blot - Anti-CHCH antibody (AB243505)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Secondary Antibodies

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise