JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB237768

Anti-CHD1L antibody

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Polyclonal CHD1L antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CHD1L aa 700 to C-terminus.

대체 명칭 보기

ALC1, CHD1L, ATP-dependent chromatin remodeler CHD1L, Amplified in liver cancer protein 1, Chromo domain-containing protein 1-like

1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-CHD1L antibody (AB237768)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CHD1L antibody (AB237768)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CHD1L (Green) using ab237768 at 1/200 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CHD1L aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q86WJ1

style
left-column

대체품

Primary Antibodies

AB240342

Anti-CHD1L antibody [EPR14515(2)] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CHD1L antibody [EPR14515(2)] - BSA and Azide free (AB240342)

  • 0
  • 0
Primary Antibodies

AB217238

Alexa Fluor® 555 Anti-CHD1L antibody [EPR14515(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-CHD1L antibody [EPR14515(2)] (AB217238)

  • 5
  • 4
Primary Antibodies

AB51324

Anti-CHD1L antibody [2170C3a]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD1L antibody [2170C3a] (AB51324)

  • 5
  • 2
Primary Antibodies

AB197019

Anti-CHD1L antibody [EPR14515(2)]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CHD1L antibody [EPR14515(2)] (AB197019)

  • 0
  • 0

보완 제품

Primary Antibodies

AB9110

Anti-HA tag antibody - ChIP Grade

Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (AB9110)

  • 5
  • 59
Primary Antibodies

AB314652

Anti-FLAP antibody [EPR27505-52]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLAP antibody [EPR27505-52] (AB314652)

  • 0
  • 0
Primary Antibodies

AB110082

Anti-FUT3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUT3 antibody (AB110082)

  • 5
  • 1
Primary Antibodies

AB181557

Anti-Oct4 antibody [EPR17929] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody [EPR17929] - ChIP Grade (AB181557)

  • 5
  • 20
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity greater than 95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The CHD1L gene encodes a protein known as chromodomain helicase DNA-binding protein 1-like. This protein plays a role in chromatin remodeling using the energy from ATP hydrolysis to alter chromatin structure thereby facilitating processes such as DNA repair and transcription. The mass of CHD1L is approximately 120 kDa. This protein is expressed in various tissues with a notable presence in liver and cancerous tissues indicating its potential involvement in cellular proliferation and repair mechanisms.
Biological function summary

The function of CHD1L is significant in genomic stability and response to DNA damage. It acts as a part of the chromatin remodeling complex and contributes to the regulation of chromatin dynamics affecting gene expression and repair pathways. CHD1L interacts with other proteins involved in DNA repair ensuring the cell maintains genetic integrity under stress conditions.

Pathways

CHD1L is involved in the DNA damage response and chromatin organization pathways. It interacts with RAD51-associated protein which is important for homologous recombination repair and collaborates with proteins in the pathway to restore DNA integrity after damage. CHD1L's activity in these pathways highlights its role in maintaining cellular homeostasis and preventing genomic instability.

CHD1L has been implicated in hepatocellular carcinoma and ovarian cancer. The overexpression of CHD1L in such cancers suggests a role in cancer progression by promoting cell proliferation and survival. Additionally its interaction with HNF4A a protein involved in liver cancer accentuates its impact on oncogenic pathways. Understanding CHD1L in these contexts could offer insights into therapeutic targets for cancer treatment.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

ATP-dependent chromatin remodeler that mediates chromatin-remodeling following DNA damage (PubMed : 19661379, PubMed : 29220652, PubMed : 29220653, PubMed : 33357431, PubMed : 34210977, PubMed : 34486521, PubMed : 34874266). Recruited to DNA damage sites through interaction with poly-ADP-ribose : specifically recognizes and binds histones that are poly-ADP-ribosylated on serine residues in response to DNA damage (PubMed : 19661379, PubMed : 29220652, PubMed : 29220653, PubMed : 34486521, PubMed : 34874266). Poly-ADP-ribose-binding activates the ATP-dependent chromatin remodeler activity, thereby regulating chromatin during DNA repair (PubMed : 19661379, PubMed : 29220652, PubMed : 29220653, PubMed : 34486521, PubMed : 34874266). Catalyzes nucleosome sliding away from DNA breaks in an ATP-dependent manner (PubMed : 19661379, PubMed : 29220652, PubMed : 29220653). Chromatin remodeling activity promotes PARP2 removal from chromatin (PubMed : 33275888).
See full target information CHD1L
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Respiratory research 24:33 PubMed36707853

2023

SLC38A6 expression in macrophages exacerbates pulmonary inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhao Peng,Weichao Chen,Fumeng Huang,Manman Geng,Xiaowei Li,Fujun Zhang,Wenhua Zhu,Liesu Meng,Rikard Holmdahl,Jing Xu,Shemin Lu
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com