Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (AB240942)

Immunohistochemical staining of paraffin-embedded Human ovary tissue using ab109195 at a dilution of 1/100.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (AB240942)

Immunofluorescent staining of HeLa cells using ab109195 at a dilution of 1/100.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (AB240942)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CHD3 with purified ab109195 at 1/230 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (AB240942)

Immunohistochemical staining of paraffin-embedded Human breast tissue using ab109195 at a dilution of 1/100.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (AB240942)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : CHD3 knockout HAP1 cell lysate (20 μg)
Lane 3 : Raji cell lysate (20 μg)
Lane 4 : Y79 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab109195).

All lanes:

Western blot - Anti-CHD3 antibody [EPNCIR110A] (<a href='/ko/products/primary-antibodies/chd3-antibody-epncir110a-ab109195'>ab109195</a>)

Predicted band size: 226 kDa

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