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AB264417

Anti-CHD4 antibody [BLR066G]

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Rabbit Recombinant Monoclonal CHD4 antibody. Suitable for IP, WB, Flow Cyt and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CHD4 aa 1-100.

대체 명칭 보기

ATP-dependent chromatin remodeler CHD4, Chromo domain-containing protein 4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, CHD-4, CHD4

4 이미지
Flow Cytometry - Anti-CHD4 antibody [BLR066G] (AB264417)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CHD4 antibody [BLR066G] (AB264417)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human CHD4 with ab264417 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

Immunoprecipitation - Anti-CHD4 antibody [BLR066G] (AB264417)
  • IP

Supplier Data

Immunoprecipitation - Anti-CHD4 antibody [BLR066G] (AB264417)

CHD4 was immunoprecipitated from 1 mg HEK-293T whole cell lysate using ab264417 at 20 μl/mg lysate. Western blot was performed on the immunoprecipitate using ab264417 at 1/1000 dilution.

Lane 1 : ab264417 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-CHD4 antibody [BLR066G] (ab264417)

Predicted band size: 218 kDa

true

Exposure time: 1s

Western blot - Anti-CHD4 antibody [BLR066G] (AB264417)
  • WB

Supplier Data

Western blot - Anti-CHD4 antibody [BLR066G] (AB264417)

All lanes:

Western blot - Anti-CHD4 antibody [BLR066G] (ab264417) at 1/1000 dilution

Lane 1:

MCF7 whole cell lysate at 50 µg

Lane 2:

A549 whole cell lysate at 50 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

K562 whole cell lysate at 50 µg

Lane 5:

Jurkat whole cell lysate at 50 µg

Lane 6:

RKO whole cell lysate at 50 µg

Secondary

All lanes:

Rabbit anti-GAPDH

Predicted band size: 218 kDa

true

Exposure time: 10s

Western blot - Anti-CHD4 antibody [BLR066G] (AB264417)
  • WB

Supplier Data

Western blot - Anti-CHD4 antibody [BLR066G] (AB264417)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-CHD4 antibody [BLR066G] (ab264417) at 1/1000 dilution

All lanes:

TCMK-1 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 218 kDa

true

Exposure time: 75s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR066G

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, Flow Cyt, IP

applications

Immunogen

Synthetic Peptide within Human CHD4 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q14839

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대체품

Primary Antibodies

AB240640

Anti-CHD4 antibody [EPR22953-38] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (AB240640)

  • 0
  • 0
Primary Antibodies

AB181370

Anti-CHD4 antibody [EPR12229]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CHD4 antibody [EPR12229] (AB181370)

  • 0
  • 0
Primary Antibodies

AB250495

Anti-CHD4 antibody [EPR12229] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CHD4 antibody [EPR12229] - BSA and Azide free (AB250495)

  • 0
  • 0
Primary Antibodies

AB263025

Anti-CHD4 antibody [EPR22953-38] - ChIP Grade - BSA and Azide free

RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade - BSA and Azide free (AB263025)

  • 0
  • 0
Primary Antibodies

AB70469

Anti-CHD4 antibody [3F2/4]

BOND RX™ Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD4 antibody [3F2/4] (AB70469)

  • 5
  • 8
Primary Antibodies

AB264521

Anti-CHD4 antibody [3F2/4] - ChIP Grade - BSA and Azide free

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD4 antibody [3F2/4] - ChIP Grade - BSA and Azide free (AB264521)

  • 0
  • 0

보완 제품

Primary Antibodies

AB8106

Anti-MTA2/PID antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody (AB8106)

  • 5
  • 2
Primary Antibodies

AB110641

Anti-BRG1 antibody [EPNCIR111A]

20ul selling size|BOND RX™ Validated|Recombinant|KO Validated|Advanced Validation|RabMAb

ChIC/CUT&RUN sequencing - Anti-BRG1 antibody [EPNCIR111A] (AB110641)

  • 5
  • 10
Primary Antibodies

AB12073

Anti-SUZ12 antibody

KO Validated

Western blot - Anti-SUZ12 antibody (AB12073)

  • 4
  • 27
Primary Antibodies

AB79416

Anti-RBBP4 antibody [EPR3411] - ChIP Grade

RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-RBBP4 antibody [EPR3411] - ChIP Grade (AB79416)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Purified from TCS.
보관 버퍼
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CHD4 also known as CDH4 is a protein that functions as part of the nucleosome remodeling and deacetylase (NuRD) complex. CHD4 has a molecular mass of approximately 218 kDa. The protein contains a chromo domain a helicase domain and two PHD-type zinc fingers. It is expressed ubiquitously in human tissues with higher expression levels seen in the spleen and fetal brain. This protein plays an essential role in DNA transcription regulation by modifying chromatin structure.
Biological function summary

CHD4 acts within the NuRD complex to mediate transcriptional repression. This complex combines chromatin remodeling with histone deacetylation to alter chromatin dynamics affecting gene expression. CHD4's involvement in the complex facilitates the static organization of chromatin therefore contributing to transcriptional silencing of specific gene sets. The protein's activity is often in response to environmental and developmental cues playing an integral role in cellular differentiation and proliferation processes.

Pathways

CHD4 connects closely with DNA damage repair and cell cycle regulation pathways. It participates actively in the DNA damage response pathway by facilitating the repair of double-strand breaks working alongside proteins such as ATM and ATR. CHD4 also influences the cell cycle by interacting with cyclins and cyclin-dependent kinases which maintain proper cell cycle progression and check points.

CHD4 has been linked to various cancers and neurodevelopmental disorders. Alterations or mutations in CHD4 have associations with certain cancers like colorectal cancer where it affects cellular proliferation and migration. In neurodevelopmental disorders disrupted CHD4 function may lead to chromatin misregulation contributing to intellectual disabilities. CHD4’s interactions with proteins such as p53 in cancer and MeCP2 in neurological conditions highlight its connections in disease mechanisms.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA (PubMed : 28977666, PubMed : 32543371). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 17626165, PubMed : 28977666, PubMed : 9804427). Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination (PubMed : 25593309). Involved in neurogenesis (By similarity).
See full target information CHD4
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 25: PubMed38928307

2024

The Immune Checkpoint BTLA in Oral Cancer: Expression Analysis and Its Correlation to Other Immune Modulators.

Applications

Unspecified application

Species

Unspecified reactive species

Jutta Ries,Leah Trumet,Alina Hahn,Lina Kunater,Rainer Lutz,Carol Geppert,Marco Kesting,Manuel Weber
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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