Anti-Chitinase 3 like protein 3 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Chitinase 3 like protein 3 antibody (AB93034)

ICC/IF image of ab93034 stained RAW 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93034 10μg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• WB

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Western blot - Anti-Chitinase 3 like protein 3 antibody (AB93034)

All lanes:

Western blot - Anti-Chitinase 3 like protein 3 antibody (ab93034) at 1 µg/mL

Lane 1:

Mouse Bone Marrow Tissue Lysate at 10 µg

Lane 2:

Spleen (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 44 kDa

Observed band size: 26 kDa,37 kDa,44 kDa

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Exposure time: 30s