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AB271961

Anti-Chk2 antibody [EPR19236] - BSA and Azide free

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Rabbit Recombinant Monoclonal Chk2 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Mouse samples.

대체 명칭 보기

Chk2, Rad53, Chek2, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Checkpoint kinase 2

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] - BSA and Azide free (AB271961)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] - BSA and Azide free (AB271961)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Chk2 with ab199031 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101, ready to use). Nuclear staining on spermatogonia of mouse testis is observed (PMID : 12192050, 11593395). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101, ready to use.

Nuclear staining on spermatogonia of mouse testis (PMID : 12192050, PMID : 11593395).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199031).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] - BSA and Azide free (AB271961)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] - BSA and Azide free (AB271961)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Chk2 with ab199031 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101, ready to use). Nuclear staining on mouse spleen is observed (PMID : 12192050, 11593395). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101, ready to use.

Nuclear staining on mouse spleen (PMID : 12192050, PMID : 11593395).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199031).

Immunoprecipitation - Anti-Chk2 antibody [EPR19236] - BSA and Azide free (AB271961)
  • IP

Lab

Immunoprecipitation - Anti-Chk2 antibody [EPR19236] - BSA and Azide free (AB271961)

Chk2 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate with ab199031 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199031 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Exposure time 3 minutes.

Lane 1 : NIH/3T3 whole cell lysate 10 μg (Input).

Lane 2 : ab199031 IP in NIH/3T3 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199031 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199031).

All lanes:

Immunoprecipitation - Anti-Chk2 antibody [EPR19236] (<a href='/ko/products/primary-antibodies/chk2-antibody-epr19236-ab199031'>ab199031</a>) at 1/1000 dilution

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

false

Exposure time: 3min

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19236

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB200762

Alexa Fluor® 488 Anti-Chk2 antibody [EPR4325]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Chk2 antibody [EPR4325] (AB200762)

  • 0
  • 0
Primary Antibodies

AB109413

Anti-Chk2 antibody [EPR4325]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR4325] (AB109413)

  • 5
  • 9
Primary Antibodies

AB207446

Anti-Chk2 antibody [EPR19482]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Chk2 antibody [EPR19482] (AB207446)

  • 5
  • 1
Primary Antibodies

AB251477

Anti-Chk2 antibody [EPR19482] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-Chk2 antibody [EPR19482] - BSA and Azide free (AB251477)

  • 0
  • 0
Primary Antibodies

AB227998

Anti-Chk2 antibody [EPR4325] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR4325] - BSA and Azide free (AB227998)

  • 0
  • 0

보완 제품

Primary Antibodies

AB32531

Anti-Chk1 antibody [E250]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Chk1 antibody [E250] (AB32531)

  • 4
  • 4
Primary Antibodies

AB81292

Anti-ATM (phospho S1981) antibody [EP1890Y]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)

  • 5
  • 18
Primary Antibodies

AB32420

Anti-ATM antibody [Y170]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM antibody [Y170] (AB32420)

  • 5
  • 8
Primary Antibodies

AB109520

Anti-p21 antibody [EPR362]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-p21 antibody [EPR362] (AB109520)

  • 5
  • 17
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

ab271961 is the carrier-free version of ab199031.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (By similarity). Under oxidative stress, promotes ATG7 ubiquitination by phosphorylating the E3 ubiquitin ligase TRIM32 at 'Ser-56' leading to positive regulation of the autophagosme assembly (By similarity).
See full target information Chek2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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