AB134964

Anti-CHM antibody [EPR7142]

Name: Anti-CHM antibody [EPR7142]
Brand: Abcam Limited
SKU: AB134964
Rating: 4 (2 reviews)

RabMAb
Recombinant
각 태그의 뜻

(2 리뷰들)

(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CHM antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-CHM antibody [EPR7142] (AB134964)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CHM antibody [EPR7142] (AB134964)

Immunofluorescence analysis of CHM in A431 cells, using ab134964 at a dilution of 1/250.

Unknown

Western blot - Anti-CHM antibody [EPR7142] (AB134964)

All lanes:

Western blot - Anti-CHM antibody [EPR7142] (ab134964) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Y79 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 73 kDa

Observed band size: 95 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7142

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purity

Tissue culture supernatant

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The CHM gene encodes for the Rab escort protein-1 (REP-1) which plays an essential role in the prenylation of Rab proteins a type of small GTPases involved in vesicle trafficking. The protein has a molecular mass of approximately 90 kDa. CHM is expressed in multiple tissues including the retina and nervous system. The alternate names for this protein include component of choroideremia and TCD which reflects its connection to cellular trafficking and transport processes.

Biological function summary

CHM is integral to the prenylation process which is important for anchoring Rab proteins to cellular membranes. Rab escort protein-1 acts within the Rab geranylgeranyltransferase complex facilitating the transfer of lipid groups to Rab proteins. This modification is necessary for proper Rab function affecting intracellular vesicle trafficking and membrane dynamics. CHM's biological actions are critical to cellular processes such as endocytosis exocytosis and autophagy.

Pathways

The CHM protein influences key cellular pathways such as the intracellular vesicle transport pathway and the autophagy pathway. Within these pathways CHM closely interacts with Rab proteins including Rab1 and Rab27 that are important in regulating vesicle movement within cells. The association of CHM with these pathways highlights its role in maintaining cellular transport and recycling mechanisms.

CHM is strongly linked to choroideremia a genetic disorder leading to progressive vision loss. Mutations in the CHM gene disrupt the normal function of REP-1 affecting the prenylation of Rab proteins and impairing cellular processes in the retina. Additionally CHM mutations can have implications in disorders related to X-linked inheritance. Research also connects CHM with pathogenic proteins like Rab27 linked to granule release disorders highlighting the impact of its malfunction in disease development.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Substrate-binding subunit of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B composed of RABGGTA and RABGGTB, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Besides, a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation.

See full target information CHM

대체 명칭 보기

REP1, TCD, CHM, Rab proteins geranylgeranyltransferase component A 1, Choroideremia protein, Rab escort protein 1, TCD protein, REP-1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The EMBO journal 39:e104958 PubMed32946136

2020

RNA localization and co-translational interactions control RAB13 GTPase function and cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Konstadinos Moissoglu,Michael Stueland,Alexander N Gasparski,Tianhong Wang,Lisa M Jenkins,Michelle L Hastings,Stavroula Mili

International journal of molecular sciences 21: PubMed32751352

2020

RhoA/ROCK Pathway Activation is Regulated by AT1 Receptor and Participates in Smooth Muscle Migration and Dedifferentiation via Promoting Actin Cytoskeleton Polymerization.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Qi,Xiuying Liang,Fan Dai,Haijing Guan,Jingwen Sun,Wenjuan Yao

Journal of neurochemistry 151:351-369 PubMed31038732

2019

Autophagic degradation of stromal interaction molecule 2 mediates disruption of neuronal dendrites by endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhou,Juan Song,Shengzhou Wu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CHM antibody [EPR7142]

Immunocytochemistry/ Immunofluorescence - Anti-CHM antibody [EPR7142] (AB134964)

Western blot - Anti-CHM antibody [EPR7142] (AB134964)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purity

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise