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AB242221

Anti-CHMP7 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal CHMP7 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CHMP7 aa 300-350.

대체 명칭 보기

Charged multivesicular body protein 7, Chromatin-modifying protein 7, CHMP7

2 이미지
Immunoprecipitation - Anti-CHMP7 antibody (AB242221)
  • IP

Supplier Data

Immunoprecipitation - Anti-CHMP7 antibody (AB242221)

CHMP7 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242221 at 20 μl/reaction.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-CHMP7 antibody (ab242221) at 1/250 dilution

Lane 1:

ab242221 IP in HEK-293T whole cell lysate

Lane 2:

Control IgG IP in HEK-293T whole cell lysate

Predicted band size: 51 kDa

true

Exposure time: 30s

Western blot - Anti-CHMP7 antibody (AB242221)
  • WB

Supplier Data

Western blot - Anti-CHMP7 antibody (AB242221)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-CHMP7 antibody (ab242221) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Predicted band size: 51 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CHMP7 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q8WUX9

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
Antibody was affinity purified using an epitope specific to CHMP7 immobilized on solid support.
보관 버퍼
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

ESCRT-III-like protein required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase (PubMed : 26040712). Together with SPAST, the ESCRT-III complex promotes NE sealing and mitotic spindle disassembly during late anaphase (PubMed : 26040712, PubMed : 28242692). Recruited to the reforming NE during anaphase by LEMD2 (PubMed : 28242692). Plays a role in the endosomal sorting pathway (PubMed : 16856878).
See full target information CHMP7

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS pathogens 21:e1013217 PubMed40445976

2025

Temporal gating of nuclear import: How Merkel cell polyomavirus exploits the cell cycle for nuclear entry.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Wang,Adrienne N Eady,Isabel Amaya,Alina Stanczak,Chelsey C Spriggs

Communications biology 6:1211 PubMed38017066

2023

Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline E Strong,Jiajing Zhang,Martin Carrasco,Srikanya Kundu,Molly Boutin,Harshad D Vishwasrao,Jiamin Liu,Angelica Medina,Yu-Chi Chen,Kelli Wilson,Emily M Lee,Marc Ferrer
제품이 사용된 논문 모두 보기

Product promise

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