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AB236742

Anti-CHODL antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal CHODL antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CHODL aa 100-250.

대체 명칭 보기

C21orf68, PRED12, UNQ872/PRO1890, CHODL, Chondrolectin, Transmembrane protein MT75

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-CHODL antibody (AB236742)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CHODL antibody (AB236742)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for CHODL (green) using ab236742 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear conterstain is DAPI (blue).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHODL antibody (AB236742)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHODL antibody (AB236742)

Paraffin-embedded human spleen tissue stained for CHODL using ab236742 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CHODL aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q9H9P2

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

May play a role in the development of the nervous system such as in neurite outgrowth and elongation. May be involved in motor axon growth and guidance.
See full target information CHODL

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 15:1420847 PubMed38975339

2024

Integrating single-cell and spatial transcriptomic analysis to unveil heterogeneity in high-grade serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haixia Luo,Kunyu Wang,Bin Li

BMC cancer 22:1364 PubMed36581816

2022

mA RNA methylation regulator-based signature for prognostic prediction and its potential immunological role in uterine corpus endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Fang,Peipei Wang,Haoyu Huang,Mingquan Ye,Xiaocen Liu,Qingqing Li

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:691-699 PubMed34118147

2021

Upregulation of centromere protein K is crucial for lung adenocarcinoma cell viability and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Yongbo Wang,Yue Wang,Chunna Ren,Haicun Wang,Yang Zhang,Yunxia Xiu
제품이 사용된 논문 모두 보기

Product promise

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