AB235938

Anti-Choline kinase alpha antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal Choline kinase alpha antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CHKA aa 50-200.

대체 명칭 보기

CHK, CKI, CHKA, Choline kinase alpha, CK, CHETK-alpha, Ethanolamine kinase, EK

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Choline kinase alpha antibody (AB235938)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Choline kinase alpha antibody (AB235938)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Choline kinase alpha (green) using ab235938 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor^® 488-congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline kinase alpha antibody (AB235938)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline kinase alpha antibody (AB235938)

Paraffin-embedded human lung cancer tissue stained for Choline kinase alpha using ab235938 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CHKA aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P35790

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Choline kinase alpha also known as CKα or CHKA is an enzyme responsible for catalyzing the first step in the synthesis of phosphatidylcholine converting choline into phosphocholine. This process is important for membrane biosynthesis and cell signaling. Choline kinase alpha has a molecular mass of approximately 45 kDa. It is expressed in various tissues with high levels in liver brain and muscle reflecting its role in both metabolic and proliferative processes. The functional activity of CKα is influenced by its interaction with lipid environments and other cellular factors.

Biological function summary

Choline kinase alpha plays a significant role in cell proliferation and survival due to its involvement in phospholipid metabolism. The enzyme is not known to be part of any large protein complexes but it does interact with a variety of cellular components that regulate its activity. It affects cellular processes by controlling the rate of phosphatidylcholine synthesis therefore impacting membrane dynamics and signal transduction. Changes in CKα activity can result in altered cell growth and function.

Pathways

Choline kinase alpha is an essential part of the Kennedy pathway or the CDP-choline pathway which is a major pathway for phosphatidylcholine biosynthesis. Within this pathway CKα's activity is essential for progressing from choline uptake to the formation of phosphatidylcholine. Other key proteins related through this pathway include CTP:phosphocholine cytidylyltransferase (CCT) and cholinephosphotransferase. The enzyme's function integrates metabolic and signaling pathways connecting lipid metabolism with cellular responses to external stimuli.

Choline kinase alpha aberrations have been linked to cancer and neurodegenerative conditions. Overexpression of CKα is frequently observed in several types of cancer highlighting its role in tumor growth and metastasis. In cancer CKα interacts with oncogenic proteins that further drive malignancy. In neurodegenerative diseases altered choline metabolism may induce neuronal damage but the exact role of CKα in such conditions needs further investigation. Targeting CKα or its pathways could provide therapeutic potential for these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a key role in phospholipid biosynthesis by catalyzing the phosphorylation of free choline to phosphocholine, the first step in phosphatidylcholine biosynthesis (PubMed : 17007874, PubMed : 19915674, PubMed : 23416529, PubMed : 34077757). Also phosphorylates ethanolamine, thereby contributing to phosphatidylethanolamine biosynthesis (PubMed : 17007874, PubMed : 19915674). Has higher activity with choline (PubMed : 17007874, PubMed : 19915674). May contribute to tumor cell growth (PubMed : 19915674).. Isoform 1. This isoform plays a key role in lipolysis of lipid droplets following glucose deprivation (PubMed : 34077757). In response to glucose deprivation, phosphorylated by AMPK, promoting localization to lipid droplets (PubMed : 34077757). Phosphorylation is followed by acetylation by KAT5, leading to dissociation of the homodimer into a monomer (PubMed : 34077757). Monomeric CHKA isoform 1 is converted into a tyrosine-protein kinase, which phosphorylates lipid droplet structural proteins PLIN2 and PLIN3, leading to lipolysis of lipid droplets (PubMed : 34077757).

See full target information CHKA

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of experimental & clinical cancer research : CR 44:112 PubMed40181456

2025

TRAF7 knockdown induces cellular senescence and synergizes with lomustine to inhibit glioma progression and recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Chen,Tongyu Zhou,Rongrong Zhou,Wen Sun,Yan Li,Qiyi Zhou,Dongcheng Xu,Yuxin Zhao,Peihao Hu,Jingrui Liang,Yumeng Zhang,Bin Zhong,Juncheng Yao,Di Jing

EMBO molecular medicine 14:e15200 PubMed36341492

2022

Blocking STAT3/5 through direct or upstream kinase targeting in leukemic cutaneous T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Helena Sorger,Saptaswa Dey,Pablo Augusto Vieyra-Garcia,Daniel Pölöske,Andrea R Teufelberger,Elvin D de Araujo,Abootaleb Sedighi,Ricarda Graf,Benjamin Spiegl,Isaac Lazzeri,Till Braun,Ines Garces de Los Fayos Alonso,Michaela Schlederer,Gerald Timelthaler,Petra Kodajova,Christine Pirker,Marta Surbek,Michael Machtinger,Thomas Graier,Isabella Perchthaler,Yi Pan,Regina Fink-Puches,Lorenzo Cerroni,Jennifer Ober,Moritz Otte,Jana D Albrecht,Gary Tin,Ayah Abdeldayem,Pimyupa Manaswiyoungkul,Olasunkanmi O Olaoye,Martin L Metzelder,Anna Orlova,Walter Berger,Marion Wobser,Jan P Nicolay,Fiona André,Van Anh Nguyen,Heidi A Neubauer,Roman Fleck,Olaf Merkel,Marco Herling,Ellen Heitzer,Patrick T Gunning,Lukas Kenner,Richard Moriggl,Peter Wolf

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Choline kinase alpha antibody

Immunocytochemistry/ Immunofluorescence - Anti-Choline kinase alpha antibody (AB235938)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline kinase alpha antibody (AB235938)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise