AB10887

Anti-CID antibody

(2 Reviews)

(14 Publications)

Rabbit Polyclonal CID antibody. Suitable for WB, ICC/IF and reacts with Drosophila melanogaster samples. Cited in 14 publications.

대체 명칭 보기

CENP-A, CG13329, cid, Histone H3-like centromeric protein cid, CENP-A homolog, Centromere identifier protein

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (AB10887)

ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (AB10887)

Drosophila S2 cells treated with ab10887 at 1 : 100 dilution and fixed with 3% PFA for 3 mins. Red = DAPI Green = ab10887

This image is courtesy of Elaine Dunleavy

Project

Western blot - Anti-CID antibody (AB10887)

All lanes:

Western blot - Anti-CID antibody (ab10887) at 1 µg/mL

All lanes:

Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) (ab27258) at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

Predicted band size: 26 kDa

Observed band size: 12 kDa,26 kDa,42 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Drosophila melanogaster

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed : 11483958, PubMed : 16839185). Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation (PubMed : 11483958, PubMed : 16839185, PubMed : 24703848). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed : 11483958, PubMed : 16839185).

See full target information cid

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제품이 사용된 논문 (14)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of cell biology 223: PubMed38180477

2024

Multimerization of a disordered kinetochore protein promotes accurate chromosome segregation by localizing a core dynein module.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica M McGory,Vikash Verma,Dylan M Barcelos,Thomas J Maresca

Cells 11: PubMed35883570

2022

Genetic Control of Kinetochore-Driven Microtubule Growth in Mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Julia V Popova,Gera A Pavlova,Alyona V Razuvaeva,Lyubov A Yarinich,Evgeniya N Andreyeva,Alina F Anders,Yuliya A Galimova,Fioranna Renda,Maria Patrizia Somma,Alexey V Pindyurin,Maurizio Gatti

Nature communications 11:2919 PubMed32522980

2020

Spt6 is a maintenance factor for centromeric CENP-A.

Applications

Unspecified application

Species

Unspecified reactive species

Georg O M Bobkov,Anming Huang,Sebastiaan J W van den Berg,Sreyoshi Mitra,Eduard Anselm,Vasiliki Lazou,Sarah Schunter,Regina Feederle,Axel Imhof,Alexandra Lusser,Lars E T Jansen,Patrick Heun

Nucleic acids research 47:10754-10770 PubMed31535131

2019

Phosphorylation of Drosophila CENP-A on serine 20 regulates protein turn-over and centromere-specific loading.

Applications

Unspecified application

Species

Unspecified reactive species

Anming Huang,Leopold Kremser,Fabian Schuler,Doris Wilflingseder,Herbert Lindner,Stephan Geley,Alexandra Lusser

PLoS genetics 15:e1008371 PubMed31527906

2019

RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Gera A Pavlova,Julia V Popova,Evgeniya N Andreyeva,Lyubov A Yarinich,Mikhail O Lebedev,Alyona V Razuvaeva,Tatiana D Dubatolova,Anastasiya L Oshchepkova,Claudia Pellacani,Maria Patrizia Somma,Alexey V Pindyurin,Maurizio Gatti

BMC biology 16:68 PubMed29907103

2018

Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Anton Strunov,Lidiya V Boldyreva,Evgeniya N Andreyeva,Gera A Pavlova,Julia V Popova,Alena V Razuvaeva,Alina F Anders,Fioranna Renda,Alexey V Pindyurin,Maurizio Gatti,Elena Kiseleva

PLoS genetics 9:e1003719 PubMed24086141

2013

Histone chaperone NAP1 mediates sister chromatid resolution by counteracting protein phosphatase 2A.

Applications

Unspecified application

Species

Unspecified reactive species

Yuri M Moshkin,Cecile M Doyen,Tsung-Wai Kan,Gillian E Chalkley,Karen Sap,Karel Bezstarosti,Jeroen A Demmers,Zeliha Ozgur,Wilfred F J van Ijcken,C Peter Verrijzer

Current biology : CB 20:2090-9 PubMed21093267

2010

Specialization of a Drosophila capping protein essential for the protection of sperm telomeres.

Applications

IHC

Species

Drosophila melanogaster

Raphaëlle Dubruille,Guillermo A Orsi,Lætitia Delabaere,Elisabeth Cortier,Pierre Couble,Gabriel A B Marais,Benjamin Loppin

PloS one 5:e10120 PubMed20396651

2010

CenH3/CID incorporation is not dependent on the chromatin assembly factor CHD1 in Drosophila.

Applications

WB, ICC/IF

Species

Drosophila melanogaster, Drosophila melanogaster

Valerie Podhraski,Beatriz Campo-Fernandez,Hildegard Wörle,Paolo Piatti,Harald Niederegger,Günther Böck,Dmitry V Fyodorov,Alexandra Lusser

The Journal of cell biology 188:335-49 PubMed20142422

2010

SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster.

Applications

ICC/IF

Species

Drosophila melanogaster

Rihui Yan,Sharon E Thomas,Jui-He Tsai,Yukihiro Yamada,Bruce D McKee

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CID antibody

Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (AB10887)

Western blot - Anti-CID antibody (AB10887)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (14)

Product promise