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AB324684

Anti-CIDE A antibody [EPR30276-574]

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Rabbit Recombinant Monoclonal CIDE A antibody. Suitable for WB and reacts with Mouse, Rat samples.
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Western blot - Anti-CIDE A antibody [EPR30276-574] (AB324684)
  • WB

Supplier Data

Western blot - Anti-CIDE A antibody [EPR30276-574] (AB324684)

Blocking and diluting buffer and concentration : QD20250827IMG35.

Low expression : spleen (PMID : 18198355).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 18509062).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 15 seconds, lanes 2-3 : 180 seconds

All lanes:

Western blot - Anti-CIDE A antibody [EPR30276-574] (ab324684) at 1/1000 dilution

Lane 1:

Rat breast tissue lysate at 50 µg

Lane 2:

Rat brown fat tissue lysate at 50 µg

Lane 3:

Rat spleen tissue lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Observed band size: 25 kDa,23 kDa,36 kDa

false

Western blot - Anti-CIDE A antibody [EPR30276-574] (AB324684)
  • WB

Supplier Data

Western blot - Anti-CIDE A antibody [EPR30276-574] (AB324684)

Blocking and diluting buffer and concentration : QD20250827IMG34.

Low expression : spleen, heart (PMID : 18198355).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 18509062).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 15 seconds, lanes 2-4 : 37 seconds

All lanes:

Western blot - Anti-CIDE A antibody [EPR30276-574] (ab324684) at 1/1000 dilution

Lane 1:

Mouse breast tissue lysate at 20 µg

Lane 2:

Mouse brown fat tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate  at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 25 kDa,23 kDa,36 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-CIDE A antibody [EPR30276-574] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30276-574

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

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Anti-UCP1 antibody [EPR20381]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (AB209483)

  • 5
  • 4
Primary Antibodies

AB196595

Anti-CIDE B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIDE B antibody (AB196595)

  • 0
  • 0
Primary Antibodies

AB106410

Anti-PRDM16 antibody

Western blot - Anti-PRDM16 antibody (AB106410)

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Primary Antibodies

AB225314

Alexa Fluor® 488 Anti-GFP antibody [EPR14104] - GFP booster

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFP antibody [EPR14104] - GFP booster (AB225314)

  • 2
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Lipid transferase that promotes unilocular lipid droplet formation by mediating lipid droplet fusion (PubMed : 18509062, PubMed : 22144693, PubMed : 26609809, PubMed : 36477540). Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage (PubMed : 18509062, PubMed : 22144693, PubMed : 26609809). Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by promoting directional net neutral lipid transfer from the smaller to larger lipid droplets (PubMed : 18509062, PubMed : 22144693, PubMed : 26609809). The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC (PubMed : 18509062, PubMed : 22144693). May also act as a CEBPB coactivator in epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein (PubMed : 22245780). By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter (PubMed : 22245780). When overexpressed, induces apoptosis; the physiological significance of its role in apoptosis is unclear (PubMed : 9564035).
See full target information Cell death activator CIDE-A

대체 명칭 보기

Lipid transferase CIDEA, Cell death activator CIDE-A, Cell death-inducing DFFA-like effector A, Cidea
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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