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AB314758

Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free

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Rabbit Recombinant Monoclonal CITED2 antibody. Carrier free. Suitable for IHC-P, WB, IP, ICC/IF, Dot and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples.

대체 명칭 보기

MRG1, CITED2, Cbp/p300-interacting transactivator 2, MSG-related protein 1, P35srj, MRG-1

14 이미지
Immunocytochemistry/ Immunofluorescence - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HT-1080 (human fibrosarcoma epithelial cell) cells labelling CITED2 with ab314757 at 1/50 (10.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing nuclear staining in HT-1080 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

Immunoprecipitation - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • IP

Supplier Data

Immunoprecipitation - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. CITED2 was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab314757 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314757 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug Lane 2 : ab314757 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314757 in K-562 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CITED2 antibody [EPR27399-62] (<a href='/ko/products/primary-antibodies/cited2-antibody-epr27399-62-ab314757'>ab314757</a>) at 1/30 dilution

All lanes:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling CITED2 with ab314757 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse cerebrum. The section was incubated with ab314757 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse pancreatic cancer tissue labeling CITED2 with ab314757 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse pancreatic cancer. The section was incubated with ab314757 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CITED2 with ab314757 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse colon. The section was incubated with ab314757 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling CITED2 with ab314757 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat colon. The section was incubated with ab314757 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling CITED2 with ab314757 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat cerebrum. The section was incubated with ab314757 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunoprecipitation - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • IP

Supplier Data

Immunoprecipitation - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. CITED2 was immunoprecipitated from 0.35 mg MEF (mouse embryo fibroblast) treated with 5 μM MG-132 for 6 hours whole cell lysate with ab314757 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314757 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : MEF (mouse embryo fibroblast) treated with 5 μM MG-132 for 6 hours whole cell lysate Lane 2 : ab314757 IP in MEF (mouse embryo fibroblast) treated with 5 μM MG-132 for 6 hours whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314757 in MEF treated with 5 μM MG-132 for 6 hours whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CITED2 antibody [EPR27399-62] (<a href='/ko/products/primary-antibodies/cited2-antibody-epr27399-62-ab314757'>ab314757</a>) at 1/30 dilution

All lanes:

MEF (mouse embryo fibroblast) treated with 5 μM MG-132 for 6 hours whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3min

Western blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • WB

Lab

Western blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CITED2 antibody [EPR27399-62] (<a href='/ko/products/primary-antibodies/cited2-antibody-epr27399-62-ab314757'>ab314757</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) + si-Scramble whole cell lysate at 20 µg

Lane 2:

HeLa + si-CITED2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa,36 kDa

false

Exposure time: 10s

Western blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • WB

Supplier Data

Western blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : Lane 1-3 : 48 seconds Lane 4 : 37 seconds

All lanes:

Western blot - Anti-CITED2 antibody [EPR27399-62] (<a href='/ko/products/primary-antibodies/cited2-antibody-epr27399-62-ab314757'>ab314757</a>) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

Western blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • WB

Supplier Data

Western blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 17906695).

Lysates were freshly made and used immediately to minimize protein degradation.

In Western Blot, Anti HIF-1 alpha antibody [EPR16897-101] (ab179483) is applied at 1/1000 dilution to prove the DFO induction is successful.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CITED2 antibody [EPR27399-62] (<a href='/ko/products/primary-antibodies/cited2-antibody-epr27399-62-ab314757'>ab314757</a>) at 1/1000 dilution

Lane 1:

Untreated HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HT-1080 treated with 0.5 mM DFO for 24 hours whole cell lysate at 20 µg

Lane 3:

HT-1080 treated with 0.5 mM DFO for 24 hours, then added 10uM MG-132 for 4 hours, whole cell lysate at 20 µg

Lane 4:

HT-1080 treated with 10uM MG-132 for 4 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa,36 kDa

false

Exposure time: 37s

Western blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • WB

Supplier Data

Western blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used immediately to minimize protein degradation

All lanes:

Western blot - Anti-CITED2 antibody [EPR27399-62] (<a href='/ko/products/primary-antibodies/cited2-antibody-epr27399-62-ab314757'>ab314757</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

Exposure time: 3min

Western blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • WB

Supplier Data

Western blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile observed is consistent with what has been described in the literature (PMID : 17906695). The band beneath the target band (30 kDa) is expected to be degradation products. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lane 1, 2 : 15 seconds Lane 3, 4 : 103 seconds

All lanes:

Western blot - Anti-CITED2 antibody [EPR27399-62] (<a href='/ko/products/primary-antibodies/cited2-antibody-epr27399-62-ab314757'>ab314757</a>) at 1/1000 dilution

Lane 1:

Untreated MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Lane 2:

MEF treated with 5 uM MG-132 for 6 hours, whole cell lysate at 20 µg

Lane 3:

Untreated PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

Lane 4:

PC-12 treated with 20 uM MG-132 for 5 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa,36 kDa

false

Dot Blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)
  • Dot

Supplier Data

Dot Blot - Anti-CITED2 antibody [EPR27399-62] - BSA and Azide free (AB314758)

This data was developed using ab314757, the same antibody clone in a different buffer formulation. Dot blot analysis of CITED2 using ab314757 at 1 : 1000 (0.517 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 48 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27399-62

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P, ICC/IF, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>Unsuitable for human IHC-P.<br>This antibody does not cross-react with human CITED1&#46;</p>

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보완 제품

Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Primary Antibodies

AB179483

Anti-HIF-1 alpha antibody [EPR16897]

20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [EPR16897] (AB179483)

  • 4
  • 9
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB108311

Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)]

BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (AB108311)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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제품 세부 정보

ab314758 is the carrier-free version of ab314757.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcriptional coactivator of the p300/CBP-mediated transcription complex. Acts as a bridge, linking TFAP2 transcription factors and the p300/CBP transcriptional coactivator complex in order to stimulate TFAP2-mediated transcriptional activation. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Stimulates the peroxisome proliferator-activated receptors PPARA transcriptional activity. Enhances estrogen-dependent transactivation mediated by estrogen receptors. Also acts as a transcriptional corepressor; interferes with the binding of the transcription factors HIF1A or STAT2 and the p300/CBP transcriptional coactivator complex. Participates in sex determination and early gonad development by stimulating transcription activation of SRY. Plays a role in controlling left-right patterning during embryogenesis; potentiates transcriptional activation of NODAL-mediated gene transcription in the left lateral plate mesoderm (LPM). Plays an essential role in differentiation of the adrenal cortex from the adrenogonadal primordium (AGP); stimulates WT1-mediated transcription activation thereby up-regulating the nuclear hormone receptor NR5A1 promoter activity. Associates with chromatin to the PITX2 P1 promoter region.
See full target information CITED2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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