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AB250213

Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CKLF antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

대체 명칭 보기

CKLF1, HSPC224, UNQ410/PRO772, CKLF, Chemokine-like factor, C32

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213)

This data was developed using ab180512, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CKLF with ab180512 at 1/100 dilution. Anti-rabbit HRP secondary antibody was used and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213)
  • WB

Supplier Data

Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213)

This data was developed using ab180512, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (<a href='/ko/products/primary-antibodies/cklf-antibody-epr119852-c-terminal-ab180512'>ab180512</a>) at 1/5000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213)
  • WB

Supplier Data

Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213)

This data was developed using ab180512, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (<a href='/ko/products/primary-antibodies/cklf-antibody-epr119852-c-terminal-ab180512'>ab180512</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

THP-1 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Unconjugated

    Anti-CKLF antibody [EPR11985(2)] - C-terminal

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11985(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB183218

Anti-TNF alpha antibody [EPR19147]

20ul selling size|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [EPR19147] (AB183218)

  • 5
  • 1
Primary Antibodies

AB76302

Anti-NF-kB p65 (phospho S536) antibody [EP2294Y]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302)

  • 4
  • 7
Primary Antibodies

AB215203

Anti-cleaved N-terminal GSDMD antibody [EPR20829-408]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (AB215203)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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제품 세부 정보

ab250213 is the carrier-free version of ab180512.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May play an important role in inflammation and regeneration of skeletal muscle (PubMed : 11415443). Essential for embryonic development (By similarity).. Isoform 1. Has chemotactic response in monocytes, neutrophils and lymphocytes (PubMed : 11415443). Binds CCR4 (PubMed : 16137713).
See full target information CKLF
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang

Frontiers in oncology 11:715552 PubMed34504794

2021

Single-Cell RNA Sequencing Reveals the Migration of Osteoclasts in Giant Cell Tumor of Bone.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Feng,Mingwei He,Xiaohong Jiang,Huijiang Liu,Tianyu Xie,Zhaojie Qin,Qian Huang,Shijie Liao,Chengsen Lin,Juliang He,Jiake Xu,Jie Ma,Yun Liu,Qingjun Wei
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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