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AB327553

Anti-Claudin 3 antibody [HL1528] - BSA and Azide free

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Rabbit Recombinant Monoclonal Claudin 3 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
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Immunocytochemistry/ Immunofluorescence - Anti-Claudin 3 antibody [HL1528] - BSA and Azide free (AB327553)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 3 antibody [HL1528] - BSA and Azide free (AB327553)

ab327553 detects Claudin 3 protein by immunofluorescent analysis.
Sample : MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green : Claudin 3 stained by ab327553 diluted at 1 : 500.
Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1 : 1000.
Blue : Fluoroshield with DAPI.

Western blot - Anti-Claudin 3 antibody [HL1528] - BSA and Azide free (AB327553)
  • WB

Supplier Data

Western blot - Anti-Claudin 3 antibody [HL1528] - BSA and Azide free (AB327553)

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ab327553 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Claudin 3 antibody [HL1528] - BSA and Azide free (ab327553) at 1/1000 dilution

Lane 1:

MCF-7 whole cell extract at 30 µg

Lane 2:

MDA-MB-231 whole cell extract at 30 µg

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1528

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

This antibody was raised against human Claudin 3 Intracellular domain.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Barrier-forming claudin. Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
See full target information CLDN3

대체 명칭 보기

C7orf1, CPETR2, CLDN3, Claudin-3, Clostridium perfringens enterotoxin receptor 2, Rat ventral prostate.1 protein homolog, CPE-R 2, CPE-receptor 2, hRVP1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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