AB75008

Anti-ClC-4 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal ClC-4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CLCN4.

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-ClC-4 antibody (AB75008)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ClC-4 antibody (AB75008)

Immunofluorescence analysis of ClC-4 in HUVEC cells, using ab75008 at a 1/500 dilution with (right image) or without (left image) the immunising peptide.

Unknown

Western blot - Anti-ClC-4 antibody (AB75008)

All lanes:

Western blot - Anti-ClC-4 antibody (ab75008) at 1/500 dilution

Lane 1:

extracts from MCF-7 cells at 30 µg

Lane 2:

extracts from MCF-7 cells at 30 µg with immunising peptide

Predicted band size: 84 kDa

Observed band size: 100 kDa,45 kDa,85 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CLCN4. The exact immunogen used to generate this antibody is proprietary information.

P51793

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ClC-4 also known as chloride channel 4 represents a member of the chloride channel family. It functions as an anion transporter facilitating the movement of chloride ions across cellular membranes. ClC-4 is expressed in various tissues including the kidney brain and skeletal muscle. The protein has a molecular weight of around 91 kDa and is encoded by the CLCN4 gene. Through its transmembrane domain operations ClC-4 helps maintain ion homeostasis and cell volume regulation.

Biological function summary

The functions of ClC-4 involve ion exchange activities important for cellular operations. It does not act alone but is part of a larger complex in particular cell types. In neuronal tissues ClC-4 contributes to the modulation of synaptic transmission and neuronal excitability. Its activity affects the electrochemical gradient across cell membranes essential for normal cellular signaling and metabolism.

Pathways

ClC-4 plays a role in regulating systemic chloride homeostasis and acid-base balance. It functions within pathways related to cell signaling and volume regulation. ClC-4 connects to pathways involving other chloride channels such as ClC-3 and ClC-5 which share complementary roles in ion transport and cellular function. This integration helps maintain the overall balance of ionic and pH levels within the body.

Mutations or dysfunctions in ClC-4 link to neurological conditions like X-linked mental retardation and epilepsy. Such diseases suggest that disruptions in chloride ion transport involve broader neural networking issues. ClC-4's involvement in these disorders intersects with proteins such as ClC-3 which also engages in maintaining neuronal chloride homeostasis. Understanding ClC-4 can provide insights into the pathophysiology of these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed : 18063579, PubMed : 23647072, PubMed : 25644381, PubMed : 27550844, PubMed : 28972156). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (PubMed : 29845874). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (PubMed : 29845874).

See full target information CLCN4

대체 명칭 보기

H(+)/Cl(-) exchange transporter 4, Chloride channel protein 4, Chloride transporter ClC-4, ClC-4, CLCN4

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cells 13: PubMed38920633

2024

The Immune Checkpoint Protein PD-L1 Regulates Ciliogenesis and Hedgehog Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ewud Agborbesong,Xiaogang Li

Aging 15:8744-8769 PubMed37671947

2023

Comprehensive analysis and experimental validation reveal elevated CLCN4 is a promising biomarker in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Wang,Jing Li,Weina Liu,Shiya Li,Yi Zhang,Yanbin Jin,Jinquan Cui

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ClC-4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ClC-4 antibody (AB75008)

Western blot - Anti-ClC-4 antibody (AB75008)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise