AB236733

Anti-CLCNKB antibody

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Polyclonal CLCNKB antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Chloride channel protein ClC-Kb aa 500 to C-terminus.

대체 명칭 보기

Chloride channel protein ClC-Kb, Chloride channel Kb, ClC-K2, CLCNKB

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCNKB antibody (AB236733)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCNKB antibody (AB236733)

Paraffin-embedded human breast cancer tissue stained for CLCNKB using ab236733 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-CLCNKB antibody (AB236733)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLCNKB antibody (AB236733)

HepG2 (human liver hepatocellular carcinoma cell line) cells labeling CLCNKB (Green) using ab236733 at 1/100 dilution in ICC/IF, followed by Alexa Fluor^® 488-congugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Chloride channel protein ClC-Kb aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P51801

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/4999", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Anion-selective channel permeable to small monovalent anions with ion selectivity for chloride > bromide > nitrate > iodide (PubMed : 11734858, PubMed : 12111250). Forms a homodimeric channel where each subunit has its own ion conduction pathway. May conduct double-barreled currents controlled by two types of gates, two fast gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously (PubMed : 11734858, PubMed : 12111250, PubMed : 16849430, PubMed : 18776122, PubMed : 19646679). Assembles with the regulatory subunit BSND/Barttin for sorting at the basolateral plasma membrane domain and functional switch to the ion conducting state. CLCNKB : BSND channels display mostly a linear current-voltage relationship controlled by common gate (PubMed : 11734858, PubMed : 12111250, PubMed : 16849430, PubMed : 18776122, PubMed : 19646679). Mediates chloride conductance along nephron segments, namely the thick ascending limb of Henle's loop, convoluted tubule and the collecting duct, contributing to the maintenance of systemic acid-base and electrolyte homeostasis (By similarity). Conducts chloride currents in the stria vascularis of the inner ear to establish the endocochlear potential necessary for normal hearing (PubMed : 15044642, PubMed : 18310267, PubMed : 19646679).

See full target information Chloride channel protein ClC-Kb

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 12:18519 PubMed36323745

2022

RhoA and vigilin are candidates for immunohistochemical markers for epithelioid malignant mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Hiratsuka,Takushi Yamamoto,Akihiko Yoshizawa,Shinya Toyokuni,Tatsuaki Tsuruyama

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CLCNKB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCNKB antibody (AB236733)

Immunocytochemistry/ Immunofluorescence - Anti-CLCNKB antibody (AB236733)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise