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AB239222

Anti-CLEC2D antibody

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(4 제품이 사용된 논문 )

Goat Polyclonal CLEC2D antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CLEC2D aa 100 to C-terminus.

대체 명칭 보기

CLAX, LLT1, OCIL, CLEC2D, C-type lectin domain family 2 member D, Lectin-like NK cell receptor, Lectin-like transcript 1, Osteoclast inhibitory lectin, LLT-1

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-CLEC2D antibody (AB239222)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLEC2D antibody (AB239222)

Paraformaldehyde fixed A431 (Human epidermoid carcinoma cell line) cells, permeabilized with 0.15% Triton, stained for CLEC2D (green) using ab239222 at 10 μg/ml in ICC/IF. Primary incubation for 1 hour followed by Alexa Fluor®488 secondary antibody (2 μg/ml). The nuclear stain is DAPI (blue).

Negative control : Non-immunized goat IgG (10 μg/ml) followed by Alexa Fluor®488 secondary antibody (2 μg/ml).

Flow Cytometry (Intracellular) - Anti-CLEC2D antibody (AB239222)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CLEC2D antibody (AB239222)

Flow cytometric analysis of paraformaldehyde fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells (blue line), permeabilized with 0.5% Triton. Primary incubation time 1 hour (10 μg/ml) followed by an Alexa-Fluor®488 secondary antibody (1 μg/ml).

IgG control : Non-immunized goat IgG (black line) followed by an Alexa-Fluor®488 secondary antibody.

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CLEC2D aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UHP7

Specificity

This antibody is expected to recognize all isoforms (NP_037401.1; NP_001004419.1; NP_001184246.1).

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
보관 버퍼
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis (PubMed : 16339513, PubMed : 20843815). Inhibits osteoclast formation (PubMed : 14753741, PubMed : 15123656). Inhibits bone resorption (PubMed : 14753741). Modulates the release of interferon-gamma (PubMed : 15104121). Binds high molecular weight sulfated glycosaminoglycans (PubMed : 15123656).
See full target information CLEC2D

제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in molecular neuroscience 18:1552819 PubMed40206188

2025

The restoration of REST inhibits reactivity of Down syndrome iPSC-derived astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tan Huang,Sharida Fakurazi,Pike-See Cheah,King-Hwa Ling

Heliyon 10:e27354 PubMed38509909

2024

C-type lectin 2D (CLEC2D) is upregulated in clear cell renal cell carcinoma (ccRCC) tissues and predicts poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huibing Li,Pengyi Zheng,Zhijun Li,Qingjiang Han,Bisheng Zhou,Kaixuan Wang

Shock (Augusta, Ga.) 61:592-600 PubMed37878490

2023

C-TYPE LECTIN-2D RECEPTOR CONTRIBUTES TO HISTONE-INDUCED VASCULAR BARRIER DYSFUNCTION DURING BURN INJURY.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyuan Yang,Ethan Zheng,Xiaoqi Sun,Amanda Reynolds,Monica Gonzalez,Juan Hernandez Villamil,Briana D Pando,David J Smith,Sarah Y Yuan,Mack H Wu

The Prostate 80:742-752 PubMed32449811

2020

Docetaxel suppresses immunotherapy efficacy of natural killer cells toward castration-resistant prostate cancer cells via altering androgen receptor-lectin-like transcript 1 signals.

Applications

Unspecified application

Species

Unspecified reactive species

Min Tang,Shenglin Gao,Lei Zhang,Bianjiang Liu,Jie Li,Zengjun Wang,Wei Zhang
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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