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AB245121

Anti-CLEC9A antibody [EPR22324] - BSA and Azide free

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(3 리뷰들)

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(2 제품이 사용된 논문 )

Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting CLEC9A in Flow Cytometry, IHC-P. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

대체 명칭 보기

CD370, UNQ9341/PRO34046, CLEC9A, C-type lectin domain family 9 member A

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Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labeling CLEC9A with ab223188 at 1/50 dilution. Cells were stained with Rabbit monoclonal IgG (ab172730) (Left) or ab223188 (Right). Then stained with Alexa Fluor® 647-conjugated anti-CD3, PE conjugated anti-HLA-DR and BV421 conjugated anti-CD141. Data shown was firstly gated out lymphocytes, then gated on viable CD3(-) HLA-DR (+) APC cells.

CLEC9A is selectively expressed on CD141+DC cells (Q2, ~0.05% in PBMC) and expression pattern was described in literature. (PMID : 20479116).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on dendritic cells (arrows) of human gastric cancer (PMID : 18408006; PMID : 20479116) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)

Immunohistochemical analysis of paraffin-embedded human thyoma tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on dendritic cells of human thymoma (PMID : 18408006; PMID : 20479116) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged CLEC9A overexpression construct, labeling CLEC9A with ab223188 at 1/500 dilution (Right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) was used as the secondary antibody at 1/2000 dilution. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (ab209101). Positive staining on dendritic cells of human tonsil (PMID : 18408006; PMID : 20479116) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22324

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB104909

Anti-CLEC9A antibody [7H11]

  • 0
  • 0
Primary Antibodies

AB104903

Anti-CLEC9A antibody [1F6]

  • 3
  • 1
Primary Antibodies

AB222794

Anti-CLEC9A antibody

Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody (AB222794)

  • 0
  • 0
Primary Antibodies

AB104910

Anti-CLEC9A antibody [8F9]

Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (AB104910)

  • 0
  • 0

보완 제품

Assay Kits

AB309389

Human CLEC9A ELISA Kit

Sandwich ELISA - Human CLEC9A ELISA Kit (AB309389)

  • 0
  • 0
Primary Antibodies

AB270797

Anti-CD1c antibody [EPR23189-196] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (AB270797)

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Primary Antibodies

AB52632

Anti-CD11c antibody [EP1347Y] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

  • 5
  • 18
Primary Antibodies

AB271053

Anti-CD208 antibody [EPR24265-8]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] (AB271053)

  • 0
  • 0
Primary Antibodies

AB131254

Anti-CD27 antibody [EPR8569]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR8569] (AB131254)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

What is this antibody validated in?
Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Other related products
We have a range of other formats of antibody clone [EPR22324] also available for your convenience: ab223188, Carrier free - ab245121, Alexa Fluor® 555 - ab313594, Alexa Fluor® 647 - ab315359

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
See full target information CLEC9A
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:6009 PubMed40593805

2025

Spatial and single cell mapping of castleman disease reveals key stromal cell types and cytokine pathways.

Applications

Unspecified application

Species

Unspecified reactive species

David Smith,Anna Eichinger,Éanna Fennell,Zijun Y Xu-Monette,Andrew Rech,Julia Wang,Eduardo Esteva,Arta Seyedian,Xiaoxu Yang,Mei Zhang,Dan Martinez,Kai Tan,Minjie Luo,Katherine J Young,Paul G Murray,Christopher Park,Boris Reizis,Vinodh Pillai

Cell 187:2817-2837.e31 PubMed38701783

2024

FLT3L governs the development of partially overlapping hematopoietic lineages in humans and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mana Momenilandi,Romain Lévy,Steicy Sobrino,Jingwei Li,Chantal Lagresle-Peyrou,Hossein Esmaeilzadeh,Antoine Fayand,Corentin Le Floc'h,Antoine Guérin,Erika Della Mina,Debra Shearer,Ottavia M Delmonte,Ahmad Yatim,Kevin Mulder,Mathieu Mancini,Darawan Rinchai,Adeline Denis,Anna-Lena Neehus,Karla Balogh,Sarah Brendle,Hassan Rokni-Zadeh,Majid Changi-Ashtiani,Yoann Seeleuthner,Caroline Deswarte,Boris Bessot,Cassandre Cremades,Marie Materna,Axel Cederholm,Masato Ogishi,Quentin Philippot,Omer Beganovic,Mania Ackermann,Margareta Wuyts,Taushif Khan,Sébastien Fouéré,Florian Herms,Johan Chanal,Boaz Palterer,Julie Bruneau,Thierry J Molina,Stéphanie Leclerc-Mercier,Jean-Luc Prétet,Leila Youssefian,Hassan Vahidnezhad,Nima Parvaneh,Kristl G Claeys,Rik Schrijvers,Marine Luka,Philippe Pérot,Jacques Fourgeaud,Céline Nourrisson,Philippe Poirier,Emmanuelle Jouanguy,Stéphanie Boisson-Dupuis,Jacinta Bustamante,Luigi D Notarangelo,Neil Christensen,Nils Landegren,Laurent Abel,Nico Marr,Emmanuelle Six,David Langlais,Tim Waterboer,Florent Ginhoux,Cindy S Ma,Stuart G Tangye,Isabelle Meyts,Nico Lachmann,Jiafen Hu,Mohammad Shahrooei,Xavier Bossuyt,Jean-Laurent Casanova,Vivien Béziat
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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