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AB79280

Anti-Clusterin antibody

5

(3 리뷰들)

|

(1 출판물)

Goat Polyclonal clusterin preproprotein antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication.
3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Clusterin antibody (AB79280)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Clusterin antibody (AB79280)

HeLa cells stained for Clusterin (green) using ab79280 at 10 μg/ml in ICC/IF. Cells were permeabilized with 0.15% Triton. DAPI was used as a nuclear counterstain.

Immunocytochemistry/ Immunofluorescence - Anti-Clusterin antibody (AB79280)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Clusterin antibody (AB79280)

Immunofluorescence analysis of paraformaldehyde fixed PC12 suspension cells, permeabilized with 0.15% Triton. Primary incubation 1hr using ab79280 (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Western blot - Anti-Clusterin antibody (AB79280)
  • WB

Unknown

Western blot - Anti-Clusterin antibody (AB79280)

The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, Circulation. 2001 Sep 18;104(12) : 1407-12.; PMID : 11560857).

All lanes:

Western blot - Anti-Clusterin antibody (ab79280) at 0.3 µg/mL

All lanes:

Rat Brain lysate (in RIPA buffer) at 35 µg

Predicted band size: 52 kDa

Observed band size: 37 kDa

false

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

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대체품

Primary Antibodies

AB92548

Anti-Clusterin antibody [EPR2911]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (AB92548)

  • 4
  • 3
Primary Antibodies

AB39991

Anti-Clusterin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Clusterin antibody (AB39991)

  • 0
  • 0
Primary Antibodies

AB239486

Anti-Clusterin antibody [C2]

Western blot - Anti-Clusterin antibody [C2] (AB239486)

  • 0
  • 0
Primary Antibodies

AB229445

Anti-Clusterin antibody [EPR2911] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] - Low endotoxin, Azide free (AB229445)

  • 0
  • 0
Primary Antibodies

AB229127

Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free (AB229127)

  • 0
  • 0
Primary Antibodies

AB69644

Anti-Clusterin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody (AB69644)

  • 5
  • 2

보완 제품

Primary Antibodies

AB92548

Anti-Clusterin antibody [EPR2911]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (AB92548)

  • 4
  • 3
Primary Antibodies

AB197029

Anti-ODF1 antibody [EPR15496]

RabMAb|Recombinant

Western blot - Anti-ODF1 antibody [EPR15496] (AB197029)

  • 0
  • 0
Primary Antibodies

AB131254

Anti-CD27 antibody [EPR8569]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR8569] (AB131254)

  • 5
  • 2
Primary Antibodies

AB313783

Anti-VGF antibody [EPR27029-60]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGF antibody [EPR27029-60] (AB313783)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab79280 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
보관 버퍼
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Clusterin also known as Apolipoprotein J (ApoJ) is a glycoprotein with a mass of approximately 75-80 kDa. Scientists find clusterin expressed widely in the human body including the brain kidney and reproductive organs. This protein exists in two isoforms a secreted form and a nuclear form each with different locations and functions within the cells.
Biological function summary

Clusterin plays multiple roles particularly in lipid transport apoptosis and cell-cell interactions. It does not form part of a larger protein complex but it acts as a molecular chaperone that helps in folding and clearance of damaged proteins. This capability allows clusterin to protect cells against stress conditions such as oxidative stress by preventing abnormal protein aggregation.

Pathways

Clusterin is involved in pathways related to cell survival and lipid metabolism. It plays a role in the insulin signaling pathway and interacts with proteins like Heat Shock Protein 70 (HSP70) to assist in protein folding and in the activation of apoptotic pathways when needed. Clusterin's function in these processes helps in maintaining cellular homeostasis.

Scientists associate clusterin with Alzheimer’s disease and cancer progression. Elevated levels of clusterin have been observed in Alzheimer's patients suggesting a role in amyloid-beta plaque formation. In cancer clusterin expression influences tumor progression and resistance to therapies. Its interaction with other proteins such as Bax modulates the apoptotic pathways impacting cancer cell survival and chemotherapy resistance.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information clusterin preproprotein
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biology open 10: PubMed34396394

2021

Dorso-ventral heterogeneity in tracheal basal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tomomi Tadokoro,Keisuke Tanaka,Shun Osakabe,Mimoko Kato,Hisato Kobayashi,Brigid L M Hogan,Hideki Taniguchi
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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