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AB89333

Anti-CNTFR antibody [MM0190-10L29]

5

(1 리뷰)

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(5 제품이 사용된 논문 )

Mouse Monoclonal CNTFR antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CNTFR.
2 이미지
Flow Cytometry - Anti-CNTFR antibody [MM0190-10L29] (AB89333)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CNTFR antibody [MM0190-10L29] (AB89333)

Overlay histogram showing SH-SY5Y cells stained with ab89333 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89333, 0.5μg/1x106 cells ) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2 (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [MM0190-10L29] (AB89333)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [MM0190-10L29] (AB89333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4μm) labelling CNTFR with ab89333. Tissue was treated with 15μg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0190-10L29

Isotype

IgG2

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CNTFR.

P26992

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대체품

Primary Antibodies

AB127425

Anti-CNTFR antibody

Western blot - Anti-CNTFR antibody (AB127425)

  • 0
  • 0
Primary Antibodies

AB203024

Anti-CNTFR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody (AB203024)

  • 0
  • 0

보완 제품

Assay Kits

AB309189

Human CNTFR ELISA Kit

SimpleStep

Sandwich ELISA - Human CNTFR ELISA Kit (AB309189)

  • 0
  • 0
Primary Antibodies

AB68153

Anti-STAT3 antibody [EPR787Y]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-STAT3 antibody [EPR787Y] (AB68153)

  • 4
  • 10
Primary Antibodies

AB267373

Anti-STAT3 (phospho Y705) antibody [EPR23968-52]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-STAT3 (phospho Y705) antibody [EPR23968-52] (AB267373)

  • 0
  • 0
Sample Prep & Detection Kits

AB269823

Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link®

Immunocytochemistry - Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (AB269823)

  • 5
  • 2
Primary Antibodies

AB202847

Anti-LIFR antibody

Flow Cytometry - Anti-LIFR antibody (AB202847)

  • 3
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/unavailable/mouse-igg2a-kappa-monoclonal-mg2a-53-low-endotoxin-azide-free-isotype-control-ab18414'>ab18414</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "15 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Binds to CNTF. The alpha subunit provides the receptor specificity. Receptor for heterodimeric neurotropic cytokine composed of CLCF1/CLC and CRLF1/CLF-1 (PubMed : 26858303). Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL6ST/GP130 and IL27RA/WSX1 (PubMed : 19386761).
See full target information CNTFR

대체 명칭 보기

Ciliary neurotrophic factor receptor subunit alpha, CNTF receptor subunit alpha, CNTFR-alpha, CNTFR
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancers 14: PubMed36497399

2022

Ciliary Neurotrophic Factor Modulates Multiple Downstream Signaling Pathways in Prostate Cancer Inhibiting Cell Invasiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Tossetta,Sonia Fantone,Rosaria Gesuita,Gaia Goteri,Martina Senzacqua,Fabio Marcheggiani,Luca Tiano,Daniela Marzioni,Roberta Mazzucchelli

Cell and tissue research 390:113-129 PubMed35794391

2022

Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Tossetta,Sonia Fantone,Elena Marinelli Busilacchi,Nicoletta Di Simone,Stefano R Giannubilo,Giovanni Scambia,Antonio Giordano,Daniela Marzioni

European journal of histochemistry : EJH 64: PubMed33131268

2020

Ciliary neurotrophic factor (CNTF) and its receptor (CNTFRα) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular study.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Fantone,Giovanni Tossetta,Rodolfo Montironi,Martina Senzacqua,Daniela Marzioni,Roberta Mazzucchelli

Cancer immunology research 8:1193-1205 PubMed32661094

2020

Chemotherapeutic Tumor Microparticles Elicit a Neutrophil Response Targeting Malignant Pleural Effusions.

Applications

Unspecified application

Species

Unspecified reactive species

Pingwei Xu,Ke Tang,Jingwei Ma,Huafeng Zhang,Dianheng Wang,Liyan Zhu,Jie Chen,Keke Wei,Jincheng Liu,Haiqing Fang,Liang Tang,Yi Zhang,Jing Xie,Yuying Liu,Rui Meng,Li Liu,Xiaorong Dong,Kunyu Yang,Gang Wu,Fei Ma,Bo Huang

Frontiers in endocrinology 10:768 PubMed31781039

2019

Biological Effects of Ciliary Neurotrophic Factor on hMADS Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Perugini,Eleonora Di Mercurio,Giovanni Tossetta,Ilenia Severi,Federica Monaco,Marcella Reguzzoni,Marco Tomasetti,Christian Dani,Saverio Cinti,Antonio Giordano
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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