Anti-COL8A1 antibody [EPR28738-178]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-COL8A1 antibody [EPR28738-178] (AB318972)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining Ly-6K with ab246486 at a 1/4000 dilution, ab318972 anti-COL8A1 used at an appropriate dilution and ab323317 anti-Oligodendrocyte Specific Protein used at an appropriate dilution.

Panel A : merged staining of anti-Ly-6K (green; Opal^™520), anti-COL8A1 (magenta; Opal^™690) and anti-Oligodendrocyte Specific Protein (gray; Opal^™570) on human testis.

Panel B : anti-Ly-6K staining spermatocytes in human testis.

Panel C : anti-COL8A1 staining basement membrane in human testis.

Panel D : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab246486, ab318972 and ab323317 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit using Opal Polymer HRP Ms + Rb secondary reagent (Ready to use). Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-COL8A1 antibody [EPR28738-178] (AB318972)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining CDCA2 with ab325345 at a 1/100 (5.16 μg/ml) dilution, ab318972 anti-COL8A1 used at a 1/2000 (0.264 μg/ml) dilution, and ab267377 anti-Wilms tumor protein used at a 1/500 (0.706 μg/ml) dilution.

Panel A : anti-CDCA2 (green; Opal™520), anti-COL8A1 (magenta; Opal™690), anti-Wilms tumor protein (gray; Opal™570) on human testis.

Panel B : anti-CDCA2 staining mitotic cells in human testis.

Panel C : anti-COL8A1 staining basement membrane in human testis.

Panel D : anti-Wilms tumor protein staining Sertoli cells in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325345, ab318972 and ab267377 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-COL8A1 antibody [EPR28738-178] (AB318972)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human breast tissue staining Hormone sensitive lipase with ab325336 at a 1/2000 (0.248 μg/ml) dilution, ab318972 anti-COL8A1 used at a 1/2000 (0.264 μg/ml) dilution, and ab180862 anti-SOX10 used at a 1/4000 (0.03 μg/ml) dilution.

Panel A : anti-Hormone sensitive lipase (green; Opal™520), anti-COL8A1 (magenta; Opal™690), anti-SOX10 (gray; Opal™570) on human breast.

Panel B : anti-Hormone sensitive lipase staining adipocytes in human breast.

Panel C : anti-COL8A1 staining basement membrane in human breast.

Panel D : anti-SOX10 staining nucleus in human breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325336, ab318972 and ab180862 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL8A1 antibody [EPR28738-178] (AB318972)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling COL8A1 with ab318972 at 1/2000 (0.264 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on human cerebrum. The section was incubated with ab318972 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL8A1 antibody [EPR28738-178] (AB318972)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling COL8A1 with ab318972 at 1/2000 (0.264 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on human placenta. The section was incubated with ab318972 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL8A1 antibody [EPR28738-178] (AB318972)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling COL8A1 with ab318972 at 1/2000 (0.264 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on stroma of human lung. The section was incubated with ab318972 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL8A1 antibody [EPR28738-178] (AB318972)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling COL8A1 with ab318972 at 1/2000 (0.264 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on stroma of human colon carcinoma. The section was incubated with ab318972 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL8A1 antibody [EPR28738-178] (AB318972)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling COL8A1 with ab318972 at 1/2000 (0.264 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on stroma of human cardiac muscle. The section was incubated with ab318972 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL8A1 antibody [EPR28738-178] (AB318972)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling COL8A1 with ab318972 at 1/2000 (0.264 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on stroma of human testis. The section was incubated with ab318972 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL8A1 antibody [EPR28738-178] (AB318972)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling COL8A1 with ab318972 at 1/2000 (0.264 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on stroma of human lung carcinoma. The section was incubated with ab318972 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Dot

Supplier Data

Dot Blot - Anti-COL8A1 antibody [EPR28738-178] (AB318972)

Dot blot analysis of COL8A1 using ab318972 at 1 : 1000 (0.527 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human COL8A2.

All lanes:

Dot Blot - Anti-COL8A1 antibody [EPR28738-178] (ab318972) at 1/1000 dilution

Lane 1:

His-tagged human COL8A1 fragment

Lane 2:

His-tagged human COL8A2 fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 81s