Anti-Collagen I antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody (AB21286)

Collagen I immunocytochemistry-immunofluorescence using Anti-Collagen I antibody ab21286. Publication image and figure legend from Tan, T., Hu, H., et al. 2019, Nature Communications PubMed 31346166.

Efficient remodeling of TSM by D-bLP-mediated photothermal effects. At 12 -h post injection of D-bLP, one tumor was exposed to 808 -nm laser at 2.5 W cm⁻² for 5 min, and the other one was not irradiated. These tumor tissues were collected at 4 h after laser irradiation for further measurements (a-e). a Variation of CAF and TAM populations in tumor with and without laser irradiation, scale bar, 100 µm. CAF were characterized by αSMA+/CD31- cells, which was denoted as cells with green signals, excluding red signals; TAM were presented as cells with green signals (F4/80). b Expression of collagen I and fibronectin in tumor with and without laser irradiation (green signals), scale bar, 100 µm. c Proportion of 4T1-GFP cancer cells (green signals) in tumor with and without laser irradiation, scale bar, 500 µm. d Histological examinations of tumors with and without laser irradiation by hematoxylin and eosin (H&E) staining, scale bar, 100 µm. Black arrow, karyopyknosis; red arrow, hemorrhage. e The quantified results of CAF, TAM, collagen I, fibronectin, and 4T1-GFP cancer cells in tumor with and without laser irradiation (n = 3). f Quantified variations of CAF, TAM, collagen I, fibronectin, and 4T1-GFP cancer cells in the D-bLP + laser-treated group at different time points after laser irradiation (n = 3). The data are means ± SD, *p < < 0.05, **p < < 0.01, n.s. not significant (ANOVA and two-tailed Student t test)

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody (AB21286)

Collagen I immunohistochemistry (frozen sections) using Anti-Collagen I antibody ab21286. Publication image and figure legend from Patra, D., DeLassus, E., et al., 2018, Biology Open, 7 PubMed 29437042.

Molecular analysis of embryonic bone development. IF for Runx2 (red; A-F), Col II THD (green, A-C), and Col I (red, J-L) in E15.5 femora. D-F are higher magnification images of A-C (magnified region outlined in A-C; shown without signals from Col II). ISH analysis for Sp7 (Osx) in E15.5 femora (G-I). Scale bars : (A-C,J-L) 100 μm; (D-F) 10 μm; (G-I) 250 μm.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody (AB21286)

Immunohistochemistry-immunofluorescence using Anti-Collagen I antibody ab21286. Publication image and figure legend from Kamata, M., Amano, H., et al. 2019, PLoS One, PubMed 30818366.

Tubulointerstitial fibrosis is less severe in BLT1-/- mice following UUO.(A) Representative images of kidney cortex from WT and BLT1-/- mice immunostained with an anti-type I collagen antibody. Scale bars = 50 μm. (B) Temporal changes in the area of immunoreactive collagen within tubulointerstitial spaces (expressed as % total area, excluding glomeruli and large vessels). Data are expressed as the mean ± SEM (n = 4 mice/group). *P<0.05 and **P<0.01, vs. WT. Expression of Col1a1 mRNA (C) and Col3a1 (D) mRNA in WT and BLT1-/- mice kidneys, as measured by real-time PCR. Data are expressed as the mean ± SEM (WT; Day 0 : n = 8, Day 1, 3, 5, 7 : n = 16 BLT1; Day 0 : n = 8, Day 1, 3, 5 : n = 16, Day 7 : n = 14 mice per group) *P<0.05 and **P<0.01, vs. WT. (D) Representative images showing S100A4 staining in WT and BLT1-/- kidneys after UUO. Scale bars = 100 μm. (E) Changes in the number of S100A4-positive cells in WT UUO and BLT1-/- UUO kidneys. (F) The number of S100A4-positive cells was significantly lower in BLT1-/- UUO kidneys. Data are expressed as the mean ± SEM (n = 4 mice/group). (G, H) Expression of mRNA encoding S100A4 (G) and αSMA (H) in UUO kidneys, as measured by real-time PCR. Data are expressed as the mean ± SEM (WT; Day 0 : n = 7, Day 1 : n = 12, Day 3, 5, 7 : n = 18 BLT1; Day 0 : n = 7, Day 1 : n = 12, Day 3, 5 : n = 16, Day 7 : n = 18 mice per group). *P<0.05 and **P<0.01, vs. WT. H, I) Expression of mRNA encoding TGF-β (I) and FGF-2 (J) in mouse kidneys after induction of UUO. Data are expressed as the mean ± SEM (TGF-β : WT; Day 0 : n = 8, Day 1 : n = 16, Day 3, 5, 7 : n = 20 BLT1; Day 0 : n = 8, Day 1 : n = 14, Day 3, 5 : n = 20, Day7 : n = 18 mice per group. FGF-2 : WT; Day 0 : n = 8, Day 1, 3, 5, 7 : n = 16 BLT1; Day 0 : n = 8, Day 1, 3, 5, 7 : n = 16 mice per group). *P<0.05 and **P<0.01, vs. WT mice.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody (AB21286)

Collagen I Immunohistochemistry-paraffin-immunofluorescence using Anti-Collagen I antibody ab21286. Publication image and figure legend from Fransén-Pettersson, N., Duarte, N., et al., 2016, PLoS One, PubMed 27441847.

The N-IF mouse develops liver fibrosis. Representative liver sections from 11 weeks old N-IF and 24αβNOD control mice stained with Sirius red. Representative images from two independent experiments with a total of six mice are shown. Scale bars are 500 μm in the overview images (left) and 100 μm in the enlarged images (right) (A). Immunofluorescence staining showing (B) collagen I (red), CD45 (cyan) and DAPI (blue); (C) MMP9 (green) and DAPI (blue) and; (D) ASMA (red) and DAPI (blue), in 12 weeks old N-IF and 24αβNOD control mouse livers. Representative images from two independent experiments with a total of six mice are shown. Scale bars are 100 μm. (E) Hydroxyproline content per mg liver tissue from N-IF and 24αβNOD mice (n = 6). Data are pooled from two independent experiments and shown as mean ± SEM ***p<0.001 by unpaired t-test.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody (AB21286)

immunohistochemistry-immunofluorescence using Anti-Collagen I antibody ab21286. Publication image and figure legend from Li, C., Zhen, G., et al., 2016, Nat Commun, PubMed 27126736.

Activation of RhoA promotes CTGF-VEGF complex formation in ECM, whereas inactivation of RhoA induces MMP3-mediated CTGF cleavage and VEGF release.(a-d) Immunofluorescence analysis of the binding of VEGF to ECM in different MSC cultures. MSCs were incubated with the indicated treatments for 7 days. Immunofluorescence staining was performed on non-permeabilized cells using antibodies against CTGF (a), fibronectin (b), collagen I (c) or VEGF (d). (e,f) MSCs were transfected with siRNA-control or siRNA-CTGF, then cultured with the indicated medium for 6 days. Western blot (WB) analysis of the cell lysates was performed using antibodies against CTGF and β-actin (e). Immunofluorescence staining was performed on non-permeabilized cells using antibodies against CTGF or VEGF (f). (g-j) WB and co-immunoprecipitation (IP) analyses of VEGF and CTGF in the overlay media of MSCs with different treatment. MSCs were incubated with the indicated treatments for 7 days. Overlay media : M1, M2, M3 and M4 were collected, respectively. Western blotting analysis of the overlay media was performed using antibodies against CTGF (g), MMP3 (h) and VEGF (i). Overlay media were subjected to IP assays using antibody against VEGF, the VEGF-associated CTGF was detected by western blotting with antibody against CTGF (j). (k,l) WB analysis of VEGF and CTGF in the ECMs of the cultured MSCs with different treatment. MSCs were incubated with the indicated treatment for 7 days. ECMs : E1, E2, E3 and E4 were collected. Western blotting analysis of the ECMs was performed using antibodies against CTGF (k) and VEGF (l). (m-o) WB and co-IP analysis of VEGF and CTGF in the overlay media of MSCs transfected with empty vector (EV) or L63RhoA. In the aliquots of the overlay medium collected from the cells overexpressed L63RhoA, 50 or 100 ng ml⁻¹ rMMP3 was added and the reactions were maintained at 37 °C for 2 h. Overlay media were subjected to immunoprecipitation assays using antibody against VEGF, the VEGF-associated CTGF was detected by western blotting with antibody against CTGF (m). Western blotting analysis of the overlay media was performed using antibodies against CTGF (n) and MMP3 (o). Scale bars, 50 μm. Data are representative of three independent experiments.

• WB

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Western blot - Anti-Collagen I antibody (AB21286)

-Collagen I western Blotting using Anti-Collagen I antibody ab21286. Publication image and figure legend from Sun, F. & Hu, K. 2016,Dis Markers, PubMed 26839446.

PAI-1 knockdown inhibits the TGF-β-promoted EMT in LA-4 cells. (a) PAI-1 mRNA level in LA-4 cells which were transfected with 20 or 40 nM PAI-1-specific siRNA (siRNA-PAI-1) or with control siRNA (siRNA-Con) for 12 hours. (b) Western blot analysis of PAI-1 in LA-4 cells which were transfected with 20 or 40 nM siRNA-PAI-1 or with siRNA-Con for 24 hours. (c) Western blot analysis of PAI-1, E-cadherin, and Collagen I in LA-4 cells which were transfected with 20 or 40 nM siRNA-PAI-1 or with siRNA-Con and were treated with 5 ng/mL TGF-β for 24 hours. (d) and (e) Relative levels of PAI-1 (d) or E-cadherin and Collagen I (e) in the siRNA-PAI-transfected and the TGF-β-treated LA-4 cells. All results were shown as values ± SE for three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, or ns : no significance.

false

• WB

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Western blot - Anti-Collagen I antibody (AB21286)

Collagen I western Blotting using Anti-Collagen I antibody ab21286. Duan, R., Hong, C. G., et al., 2024 BMC Pulmonary Medicine, PubMed 38178092.

Administration of OM-MSCs downregulates the expression of fibrotic factors from BLM-induced pulmonary fibrosis. (A-B) Representative images and quantification of COL1A1 staining of lung tissues from control mice or BLM-induced mice with or without OM-MSCs treatment. Scale bar : 200 mm. n = 4 per group. (C-D) Representative images and quantification of ACTA2 staining of lung tissues from control mice or BLM-induced mice with or without OM-MSCs treatment. Scale bar : 200 mm. n = 4 per group. (E-F) Representative images and quantification of MMP13 staining of lung tissues from control mice or BLM-induced mice with or without OM-MSCs treatment. Scale bar : 200 mm. n = 4 per group. (G) Measurement of hydroxyproline depositions in lung tissue of control mice or BLM-induced mice with or without OM-MSC treatment. n = 4 per group. (H-K) Representative western blot analysis and quantification of COL1A1, MMP13, ACTA2. n = 3 per group. Full-length blots/gels are presented in Supplementary file 2. (L-N) QPCR analysis of Col1a1, Mmp13, Acta2. n = 6 per group

false

• WB

CiteAb

Western blot - Anti-Collagen I antibody (AB21286)

Western Blotting using Anti-Collagen I antibody, ab21286. Publication image from Mori, M. et al., 2016, Nat Commun, 27527664. Legend direct from paper.

Mkx promotes the differentiation of mesenchymal stem cells to ligament cells.(a) Images of C3H10T1/2 cells induced by Venus (C3H10T1/2-V or Venus) and Venus-Mkx (C3H10T1/2-VM or Venus-Mkx) in plate culture. Scale bars, 500 µm (top); 100 µm (bottom). (b–e) Quantitative real-time reverse transcription PCR (qRT–PCR) analyses of the expression of ligament, cartilage, bone and fat-related genes in C3H10T1/2-V and C3H10T1/2-VM cells. mRNA levels in C3H10T1/2-V cells were normalized to 1. Error bars represent s.e.m. *P<0.05, **P<0.01, ***P<0.001. Statistical differences were assessed with Student's t-test. (f) Western blot analyses of C3H10T1/2-V and C3H10T1/2-VM cells for lineage markers.

false