AB6308

Anti-Collagen I antibody [COL-1]

Name: Anti-Collagen I 항체 [COL-1] (ab6308) | Abcam
Brand: Abcam Limited
SKU: AB6308
Rating: 4 (34 reviews)

Anti-Collagen I 항체 [COL-1]

Lab Essentials
각 태그의 뜻

(34 리뷰들)

(549 제품이 사용된 논문 )

Anti-Collagen I antibody [COL-1] (ab6308) is a mouse monoclonal antibody detecting Collagen I in Western Blot, IHC-Fr. Suitable for Human.

- Over 460 publications
- Trusted since 2000

대체 명칭 보기

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

4 이미지

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (AB6308)

IHC-Fr

AbReview2615****

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (AB6308)

Immunoperoxidase staining of unfixed frozen tissue sections with ab6308. Picture of human kidney cortex showing two glomeruli and surrounding tubulointerstitium.

This image was kindly supplied as part of the review submitted by Arvi-Matti Kuusniemi

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (AB6308)

Acetone-fixed human tonsil tissue frozen section stained for Collagen I with ab6308 at 7 μg/mL in immunohistochemical analysis.

AbReview17126****

Western blot - Anti-Collagen I antibody [COL-1] (AB6308)

All lanes:

Western blot - Anti-Collagen I antibody [COL-1] (ab6308) at 1/1000 dilution

All lanes:

Human brain whole tissue lysate at 10 µg

Secondary

All lanes:

An HRP-conjugated goat polyclonal to mouse IgG at 1/7500 dilution

Predicted band size: 139 kDa

true

Exposure time: 24hr

This image is courtesy of an anonymous Abreview

Supplier Data

Western blot - Anti-Collagen I antibody [COL-1] (AB6308)

Lane 1:

Western blot - Anti-Collagen I antibody [COL-1] (ab6308) at 2 µg/mL

Lane 2:

Western blot - Anti-Collagen I antibody [COL-1] (ab6308) at 1 µg/mL

Lane 3:

Western blot - Anti-Collagen I antibody [COL-1] (ab6308) at 0.5 µg/mL

Lane 4:

Western blot - Anti-Collagen I antibody [COL-1] (ab6308)

All lanes:

Recombinant human Collagen 1

Predicted band size: 139 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

COL-1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-Fr

applications

Immunogen

Native Full Length Protein corresponding to Cow COL1A1.

P02453

Epitope

The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding. There have been varying results when using this antibody in IHC-P. Please refer to our customer Abreviews for more protocol information and optimization steps when using this antibody in IHC-P.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions.Positive Control: Hu stomach, skin and adrenal gland tissue lysates.Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "3.5-7 µg/mL", "IHCFr-species-notes": "(amplification required).Use on unfixed tissue or acetone fixed tissue." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Deer": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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제품 세부 정보

Anti-Collagen I antibody [COL-1] (ab6308) is a House Mouse Monoclonal antibody and is validated for use in IHC-Fr, WB.

Anti-Collagen I antibody [COL-1] (ab6308) has been cited over 467 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality validation processes ensure Anti-Collagen I antibody [COL-1] (ab6308) has high sensitivity and specificity.

Anti-Collagen I antibody [COL-1] (ab6308) has 33 independent reviews from customers.

Anti-Collagen I antibody [COL-1] (ab6308) specifically detects Collagen I (UniProt ID: P02452; Molecular weight: 95kDa) and is sold in 50 uL selling sizes.

Collagen I (COL1A1)is a major component of the extracellular matrix (ECM) and plays a significant role in cancer progression. In the tumor microenvironment, Collagen I can promote tumor growth, invasion, and metastasis by increasing tissue stiffness and facilitating cancer cell migration.

Production of this antibody has been changed on 23^rd June 2016. The following lots are from ascites and are still in stock as of 23^rd June 2016 : GR210978, GR175242, GR158374. Lot numbers higher than GR210978 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Proprietary technique

Purification 관련 사항

Purified from Tissue culture supernatant.

보관 버퍼

pH: 7.4 Preservative: 0.0976% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.

Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Type I collagen is a member of group I collagen (fibrillar forming collagen).

See full target information COL1A1

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제품이 사용된 논문 (549)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cellular and molecular medicine 29:e70800 PubMed40845179

2025

Decoy Receptor 2 as a Cell Cycle Arrest Biomarker for Predicting Renal Recovery Following Acute Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangling Yi,Liming Wang,Xiao-Yue Wang,Yu Fang,Jiarui Liu,Kehong Chen,Ya-Ni He,Jia Chen

Stem cells international 2025:5572221 PubMed40822849

2025

KLF5 Regulation of Exosome-Derived miR-152-3p From Bone Marrow Stem Cells Improves Ventricular Arrhythmia After Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Wu,Xin-Yue Zou,Yi-Wen Jiang,Da-Wei Lin,Feng Jiang,Yao-Sheng Wang

Frontiers in immunology 16:1618012 PubMed40821844

2025

CRIF1 gene therapy ameliorates inflammatory bowel disease by suppressing TH17 cells and fibrosis through mitochondrial function regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Sil Park,Hye Yeon Kang,Ha Yeon Jeong,SeungCheon Yang,JeongWon Choi,Sang Hee Cho,Sol Kim,Bo-In Lee,Mi-La Cho

Frontiers in bioengineering and biotechnology 13:1615650 PubMed40791857

2025

Restoration of tendon repair microenvironment by grapefruit exosome-loaded microneedle system for tendinopathy therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Ruiyang Zhang,Ti Zhang,Yuhao Mu,Talante Juma,Xu Li,Hao Li,Quanyi Guo,Yongping Cao

JCI insight 10: PubMed40627450

2025

CAR-engineered cytolytic Tregs reverse pulmonary fibrosis and remodel the fibrotic niche with limited CRS.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Han Jiang,Meng Zhou,Meng-Di Cheng,Sai Chen,Ying-Qiang Guo

Bioactive materials 50:364-381 PubMed40589547

2025

In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Ye Huang,Xu Hu,Yulei Mu,Huiqun Zhou,Liang Ma,Bangheng Liu,Hang Yao,Xieyuan Jiang,Dong-An Wang

Pharmaceuticals (Basel, Switzerland) 18: PubMed40573271

2025

Topical Application of a Collagen Mimetic Peptide Restores Peripapillary Scleral Stiffness Reduced by Ocular Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren K Wareham,Ghazi O Bou Ghanem,Kristin L Clark,Eric Schlumpf,Brian J Del Buono,David J Calkins

International journal of molecular sciences 26: PubMed40429861

2025

Biochemical and Biomechanical Properties of Scaffold-Free Hyaline Cartilage Generated Under Dynamic Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando P S Guastaldi,David M Kostyra,Nichaluk Leartprapun,Seemantini Nadkarni,Mark A Randolph,Robert W Redmond

Bioengineering (Basel, Switzerland) 12: PubMed40428065

2025

Bilayer Type I Atelocollagen Scaffolds for In Vivo Regeneration of Articular Cartilage Defects.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Hun Woo,Bo Keun Lee,Andrew S Kwak,Jin Hyo Yang,Seo Yeon Kim,Man Soo Kim,Ji Chul Yoo

Cell and tissue research 401:129-143 PubMed40387922

2025

The effect of macromolecular crowders on deposition of extracellular matrix in astrocyte cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Sorour Nemati,Michelle Kilcoyne,Dimitrios Zeugolis,Siobhan S McMahon

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen I antibody [COL-1]

Anti-Collagen I 항체 [COL-1]

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (AB6308)

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (AB6308)

Western blot - Anti-Collagen I antibody [COL-1] (AB6308)

Western blot - Anti-Collagen I antibody [COL-1] (AB6308)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (549)

Product promise