AB235296

Anti-Collagen IV antibody

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(3 제품이 사용된 논문 )

Goat Polyclonal COL4A1 antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse samples. Cited in 3 publications.

대체 명칭 보기

Collagen alpha-1(IV) chain, Col4a1

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB235296)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB235296)

Immunohistochemical analysis of mouse placenta tissue labeling Collagen IV with ab235296 at 1/100 dilution for 30 mins at RT, followed by a donkey anti-goat HRP at 4 μL/ml for 20 mins at RT. Counterstained with hematoxylin. HIER was performed using citrate buffer for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Collagen IV antibody (AB235296)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Collagen IV antibody (AB235296)

Immunocytochemical analysis of 4% PFA-fixed mouse NIH/3T3 cells labeling Collagen IV with ab235296 at 15 μg/ml overnight at 4°C, followed by a donkey anti-goat IgG DyLight™ 488 conjugated preabsorbed secondary at 5 μg/ml for 1 hour at RT (green). The nuclear counter stain is DAPI (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB235296)

Immunohistochemical analysis of mouse testis tissue labeling Collagen IV with ab235296 at 1/100 dilution for 30 mins at RT, followed by a donkey anti-goat HRP at 4 μL/ml for 20 mins at RT. Counterstained with hematoxylin. HIER was performed using citrate buffer for 20 mins.

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB86042

Anti-Collagen IV antibody [1043]

Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [1043] (AB86042)

Primary Antibodies

AB214417

Anti-Collagen IV antibody [EPR20966]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [EPR20966] (AB214417)

Primary Antibodies

AB236640

Anti-Collagen IV antibody [EPR22911-127]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Collagen IV antibody [EPR22911-127] (AB236640)

Primary Antibodies

AB256353

Anti-Collagen IV antibody [EPR22911-127] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|Advanced Validation

Multiplex immunohistochemistry - Anti-Collagen IV antibody [EPR22911-127] - BSA and Azide free (AB256353)

Primary Antibodies

AB226485

Anti-Collagen IV antibody [EPR20966] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [EPR20966] - BSA and Azide free (AB226485)

Primary Antibodies

AB6311

Anti-Collagen IV antibody [COL-94]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (AB6311)

보완 제품

Assay Kits

AB222942

Total Collagen Assay Kit (Perchlorate-Free)

Functional Studies - Total Collagen Assay Kit (Perchlorate-Free) (AB222942)

Reagents, Controls & Accessories

AB246832

Picro-Sirius Red Solution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro-Sirius Red Solution (AB246832)

Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

Primary Antibodies

AB11575

Anti-Laminin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)

Primary Antibodies

AB7778

Anti-Collagen III antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Collagen IV also known as COL4A1 or collagen type IV is a structural protein essential to the basement membrane in tissues. The molecular weight of collagen type 4 varies but it generally forms a high-molecular-weight complex. This protein expresses prominently in various tissues especially in the kidneys lens capsule of the eye and skin. Collagen IV comprises six different alpha chains where its distinctiveness lies in its non-fibrillar network-forming structure which serves as a supportive scaffold for tissues and contributes to tissue regeneration and cell adhesion.

Biological function summary

The highly organized network of collagen type IV plays a supreme role in maintaining the integrity and functionality of the basement membrane. It exists predominantly as part of a supramolecular complex associating with laminin nidogen and perlecan forming a multifunctional platform. This platform facilitates cellular signaling tissue elasticity and filtration functions. The basement membrane's selective permeability heavily relies on this network structure to regulate the exchange of molecules between tissues like blood vessels and epithelial sheets.

Pathways

Collagen IV contributes to several critical functions within the extracellular matrix organization and cell-matrix adhesion pathways. Specifically it interacts intimately with integrins and matrix metalloproteinases (MMPs) playing roles in angiogenesis and tissue repair. Additionally its interactions with other collagens and integrins within these pathways enhance cellular responses to mechanical stress and contribute to structural resilience in tissues.

Mutations or abnormalities in collagen IV are linked closely with hereditary conditions such as Alport syndrome and Goodpasture syndrome. These conditions often result in compromised structural integrity and function of the basement membrane particularly affecting the kidneys and lungs. Alterations in collagen IV can influence interactions with other proteins like collagen type IV-associated proteins altering normal physiological functions and leading to tissue dysfunction and disease manifestations.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.

See full target information Col4a1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PloS one 20:e0322721 PubMed40694571

2025

Busulfan damages spermatogenic function by inducing orchitis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingjun Zhao,Kaihui Wu,Shiyuan Xu,Songqi Liu,Kaimin Yuan,Dong Wang

Frontiers in cardiovascular medicine 11:1319164 PubMed38545339

2024

The gene variant governs passive ascending aortic mechanics in the mgΔ mouse model of Marfan syndrome when superimposed to perlecan haploinsufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Samar A Tarraf,Rodrigo Barbosa de Souza,Ashley Herrick,Lygia V Pereira,Chiara Bellini

The American journal of pathology 190:1943-1959 PubMed32562655

2020

Acoustic Trauma Causes Cochlear Pericyte-to-Myofibroblast-Like Cell Transformation and Vascular Degeneration, and Transplantation of New Pericytes Prevents Vascular Atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Hou,Lingling Neng,Jinhui Zhang,Jing Cai,Xiaohan Wang,Yunpei Zhang,Ivan A Lopez,Xiaorui Shi

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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