Anti-Collagen IV antibody [EPR22911-127]
- BOND RX™ Validated
- RabMAb
- Recombinant
- 20ul selling size
- Advanced Validation
- 각 태그의 뜻
5
(2 리뷰들)
|
(28 제품이 사용된 논문 )
Anti-Collagen IV antibody [EPR22911-127] (ab236640) is a rabbit monoclonal antibody detecting Collagen IV in IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
대체 명칭 보기
Collagen alpha-1(IV) chain, COL4A1
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [EPR22911-127] (AB236640)
Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Collagen IV with ab236640 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining the blood vessels in human glioma (PMID : 20063114). The section was incubated with ab236640 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [EPR22911-127] (AB236640)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Collagen IV with ab236640 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Basement membrane staining on human kidney (PMID : 26260163). The section was incubated with ab236640 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
- mIHC
Lab
Multiplex immunohistochemistry - Anti-Collagen IV antibody [EPR22911-127] (AB236640)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining Collagen IV with ab236640 at a 1/1000 dilution, ab199975 anti-Aquaporin 2 used at 1/1000 dilution and ab316191 anti-SLC12A3 used at a 1/1000 dilution.
- IHC-Fr
Unknown
Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [EPR22911-127] (AB236640)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Collagen IV with ab236640 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on tubular basement membrane of mouse kidney (PMID : 23757342) is observed. Counterstained with DAPI (blue). Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
- IHC-Fr
Unknown
Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [EPR22911-127] (AB236640)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Collagen IV with ab236640 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on tubular basement membrane of rat kidney (PMID : 23757342) is observed. Counterstained with DAPI (blue). Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [EPR22911-127] (AB236640)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Collagen IV with ab236640 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Basement membrane staining on mouse kidney (PMID : 26260163). The section was incubated with ab236640 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
- mIHC
Lab
Multiplex immunohistochemistry - Anti-Collagen IV antibody [EPR22911-127] (AB236640)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining Collagen IV with ab236640 at a 1/1000 dilution, ab199975 anti-Aquaporin 2 used at 1/1000 dilution and ab316191 anti-SLC12A3 used at a 1/1000 dilution.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [EPR22911-127] (AB236640)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Collagen IV with ab236640 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the blood vessels in mouse cerebrum (PMID : 20063114). The section was incubated with ab236640 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
- mIHC
Lab
Multiplex immunohistochemistry - Anti-Collagen IV antibody [EPR22911-127] (AB236640)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat kidney tissue staining Collagen IV with ab236640 at a 1/1000 dilution, ab199975 anti-Aquaporin 2 used at 1/1000 dilution and ab316191 anti-SLC12A3 used at a 1/1000 dilution.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [EPR22911-127] (AB236640)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Collagen IV with ab236640 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Basement membrane staining on rat kidney (PMID : 26260163). The section was incubated with ab236640 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
관련 conjugated 항체와 다양한 조성의 항체 (3)
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Anti-Collagen IV antibody [EPR22911-127] - BSA and Azide free
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Collagen IV antibody [EPR22911-127]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Collagen IV antibody [EPR22911-127]
Reactivity 정보
제품 세부 정보
What is this antibody validated in?
Anti-Collagen IV antibody [EPR22911-127] (ab236640) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.
Trusted by the scientific community
Anti-Collagen IV [EPR22911-127] (ab236640) was first used in a scientific publication in 2019 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR22911-127] also available for your convenience: Alexa Fluor® 488 - ab309502, Alexa Fluor® 647 - ab309503
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The highly organized network of collagen type IV plays a supreme role in maintaining the integrity and functionality of the basement membrane. It exists predominantly as part of a supramolecular complex associating with laminin nidogen and perlecan forming a multifunctional platform. This platform facilitates cellular signaling tissue elasticity and filtration functions. The basement membrane's selective permeability heavily relies on this network structure to regulate the exchange of molecules between tissues like blood vessels and epithelial sheets.
Pathways
Collagen IV contributes to several critical functions within the extracellular matrix organization and cell-matrix adhesion pathways. Specifically it interacts intimately with integrins and matrix metalloproteinases (MMPs) playing roles in angiogenesis and tissue repair. Additionally its interactions with other collagens and integrins within these pathways enhance cellular responses to mechanical stress and contribute to structural resilience in tissues.
제품 프로토콜
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타겟 정보
제품이 사용된 논문 (28)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Journal of nanobiotechnology 23:649 PubMed41074120
2025
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Frontiers in bioengineering and biotechnology 13:1578467 PubMed40539096
2025
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Journal of ovarian research 18:126 PubMed40495228
2025
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Molecular medicine reports 31: PubMed40052569
2025
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Renal failure 47:2458764 PubMed39901448
2025
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Molecular medicine (Cambridge, Mass.) 30:231 PubMed39592929
2024
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Brain, behavior, and immunity 121:365-383 PubMed39084541
2024
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Translational vision science & technology 13:16 PubMed39042048
2024
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Heliyon 10:e33585 PubMed39040273
2024
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JCI insight 9: PubMed38713515
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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