AB260037

Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38]

Name: Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38]
Brand: Abcam Limited
SKU: AB260037
Rating: 4 (1 reviews)

RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(1 출판물)

Rabbit Recombinant Monoclonal COMP/Cartilage oligomeric matrix protein antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

대체 명칭 보기

Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5

4 이미지

Unknown

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg human articular cartilage lysate 10ug with ab260037 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260037 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human articular cartilage lysate 10ug

Lane 2 : ab260037 IP in human articular cartilage lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab260037 in human articular cartilage lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)

Predicted band size: 82 kDa

false

Unknown

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg mouse articular cartilage lysate 10ug with ab260037 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260037 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse articular cartilage lysate 10ug

Lane 2 : ab260037 IP in mouse articular cartilage lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab260037 in mouse articular cartilage lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)

Predicted band size: 82 kDa

false

Lab

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9143347, 24917676).

Negative control : Jurkat (Database : EBI Expression Atlas).

Exposure time : Lanes 1-2 : 1 second; Lane 3 : 125 seconds.

All lanes:

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037) at 1/5000 dilution

Lane 1:

Human articular cartilage lysate at 20 µg

Lane 2:

Jurkat (human t cell leukemia t lymphocyte) whole cell lysate at 20 µg

Lane 3:

Mouse articular cartilage lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 100 kDa,250 kDa

false

Lab

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9143347, 24917676).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037) at 1/5000 dilution

All lanes:

Rat articular cartilage lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 100 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22857-38

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Cartilage Oligomeric Matrix Protein (COMP) also known as oligomeric protein or COMP cartilage protein is a structural component found in the extracellular matrix of cartilage tissues. This matrix protein has a mass of approximately 524 kDa and is predominantly expressed in cartilage particularly hyaline cartilage. COMP forms pentameric structures which make it an important supporting element in the stability and resilience of cartilage. Researchers often use techniques like COMP ELISA to quantify its levels in biological samples.

Biological function summary

COMP interacts with other extracellular matrix components to enhance structural integrity and function of cartilage tissue. It performs significant roles in the organization and maintenance of the matrix tethering fibrillar proteins and supporting cellular interaction. COMP is not typically part of a large complex but works closely with collagens and other matrix proteins to form a robust and flexible extracellular environment.

Pathways

COMP participates in pathways associated with cartilage formation and turnover. It engages in chondrocyte-mediated processes that are vital for endochondral ossification and cartilage repair. Within these pathways it interacts with proteins such as collagen type II and decorin which facilitate the organization and mineralization of cartilage matrix. These interactions highlight the protein's importance in maintaining cartilage architecture and function.

COMP plays a role in chondrodysplasias and osteoarthritis. Mutations in the COMP gene can lead to skeletal disorders like pseudoachondroplasia and multiple epiphyseal dysplasia causing growth and developmental anomalies. In osteoarthritis alterations in COMP levels may serve as an indicator of cartilage degradation. COMP's relationships with proteins like aggrecan and matrilin-3 in these contexts highlight its involvement in pathologies that affect cartilage structure and health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed : 16051604, PubMed : 16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed : 16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed : 17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).

See full target information COMP

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of biochemical and molecular toxicology 35:e22848 PubMed34328678

2021

LINC00511 facilitates Temozolomide resistance of glioblastoma cells via sponging miR-126-5p and activating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Lu,Meng Tian,Jiongbo Liu,Kuanhong Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38]

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise