JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB273632

Anti-Complement C8 antibody [EPR23646-22] - BSA and Azide free

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal CO8A antibody. Carrier free. Suitable for WB and reacts with Human samples.

대체 명칭 보기

Complement component C8 alpha chain, Complement component 8 subunit alpha, C8A

1 이미지
Western blot - Anti-Complement C8 antibody [EPR23646-22] - BSA and Azide free (AB273632)
  • WB

Lab

Western blot - Anti-Complement C8 antibody [EPR23646-22] - BSA and Azide free (AB273632)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab273626).

All lanes:

Western blot - Anti-Complement C8 antibody [EPR23646-22] (<a href='/ko/products/primary-antibodies/complement-c8-antibody-epr23646-22-ab273626'>ab273626</a>) at 1/1000 dilution

All lanes:

Human plasma at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 65 kDa

Observed band size: 65 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23646-22

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<strong>This antibody specifically recognizes the Complement component C8 alpha chain.</strong>

style
left-column

보완 제품

Assay Kits

AB137971

Human Complement C8 ELISA Kit

Sandwich ELISA - Human Complement C8 ELISA Kit (AB137971)

  • 0
  • 0
Primary Antibodies

AB168345

Anti-C9 antibody [EPR11232]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (AB168345)

  • 0
  • 0
Primary Antibodies

AB278045

Anti-Complement factor 8 beta/C8B antibody [EPR23764-1]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] (AB278045)

  • 0
  • 0
Primary Antibodies

AB250407

Anti-C8G antibody [EPR11274] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - BSA and Azide free (AB250407)

  • 0
  • 0
Primary Antibodies

AB66768

Anti-C5b-9 + C5b-8 antibody [aE11]

Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (AB66768)

  • 5
  • 1
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

제품 세부 정보

ab273632 is the carrier-free version of ab273626.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Complement C8 protein plays an important role in the immune response specifically as a component of the membrane attack complex (MAC). Known also simply as C8 it is composed of three subunits: C8α C8β and C8γ with a combined molecular mass of approximately 151 kDa. C8 is synthesized primarily by liver hepatocytes but its expression occurs in several other tissues such as monocytes and macrophages highlighting its significance in immune surveillance.
Biological function summary

The membrane attack complex is a pivotal element of the complement system where Complement C8 serves as a final assembly participant. It collaborates with other proteins such as C5b C6 and C7 to penetrate and lyse target cell membranes protecting the host against pathogenic invaders. C8 forms part of a larger complement cascade which activates sequentially ensuring swift response to microbial threats. This process highlights its relevance in innate immunity.

Pathways

Several significant biological processes involve Complement C8. The complement system and the coagulation pathway both rely on this protein's function. Within these pathways C8 associates closely with proteins such as C9 in forming the lytic pore of the MAC and C3b which facilitates the opsonization and clearance of pathogens. These collaborative actions support efficient immune defense and maintain homeostatic balance within the bloodstream.

Complement system dysregulation can link to immune complex diseases such as systemic lupus erythematosus (SLE) and paroxysmal nocturnal hemoglobinuria (PNH). In SLE improper complement activation possibly involving Complement C8 may lead to the formation of immune complexes that cause inflammation and tissue damage. In PNH the lack of complement inhibitory proteins results in red blood cell destruction where the unregulated MAC formation involving C8 plays a part in the hemolytic process. Understanding these connections helps unravel the complex roles that Complement C8 has in disease pathogenesis.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself.
See full target information C8A
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com