AB278049

Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CO8B antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Recombinant full length protein - Human samples.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

This data was developed using ab278045, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Complement factor 8 beta/C8B with ab278045 at 1/1000 (0.533 μg/ml) dilution followed by a ready to use secondary antibody. Cytoplasmic staining on human liver. The section was incubated with ab278045 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use secondary antibody).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

This data was developed using ab278045, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling Complement factor 8 beta/C8B with ab278045 at 1/1000 (0.533 μg/ml) dilution followed by a ready to use secondary antibody. Positive staining on the blood plasma in human thyroid (PMID : 9476128). The section was incubated with ab278045 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use secondary antibody.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

This data was developed using ab278045, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Complement factor 8 beta/C8B with ab278045 at 1/1000 (0.533 μg/ml) dilution followed by a ready to use secondary antibody. The section was incubated with ab278045 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Negative control : no staining on human cerebrum.

Secondary antibody only control : Secondary antibody is a ready to use secondary antibody.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Supplier Data

Immunoprecipitation - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

This data was developed using ab278045, the same antibody clone in a different buffer formulation.

Complement factor 8 beta/C8B was immunoprecipitated from 0.35 mg human kidney tissue lysate with ab278045 at 1/30 dilution (2μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab278045 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human kidney tissue lysate 10 μg

Lane 2 : ab278045 IP in human kidney tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab278045 in human kidney tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] (<a href='/ko/products/primary-antibodies/complement-factor-8-beta-c8b-antibody-epr23764-1-ab278045'>ab278045</a>)

Predicted band size: 67 kDa

Observed band size: 67 kDa

false

Lab

Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

This data was developed using ab278045, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 21750743).

Negative : human brain (PMID : 16483657, PMID : 27005612).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] (<a href='/ko/products/primary-antibodies/complement-factor-8-beta-c8b-antibody-epr23764-1-ab278045'>ab278045</a>) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human large intestine tissue lysate at 20 µg

Lane 4:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

false

Lab

Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

This data was developed using ab278045, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody has no cross reaction with human C8A and C9.

Sample loaded onto lane 1 was purified protein extracted from HEK293 expressing C8B, which contains a LIF-tag (40 kDa).

C8A and C9 recombinant proteins were expressed in E.coil expression systems.

Exposure time : 26 seconds.

All lanes:

Lane 1:

LIF-tagged human C8B recombinant protein, 30 ng

Lane 2:

E.coli extracts containing his-tagged human C8A recombinant protein, 30 ng

Lane 3:

E.coli extracts containing his-tagged human C9 recombinant protein, 30 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 67 kDa

false

Lab

Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

This data was developed using ab278045, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 21750743).

Exposure time : 15 seconds.

All lanes:

Human serum at 20 µL

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23764-1

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab278049 is the carrier-free version of ab278045.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Complement factor 8 beta (C8B) is an essential protein component of the complement system an important part of the immune response. Commonly referred to as C8B this protein has a molecular mass of approximately 64 kilodaltons. C8B is primarily expressed in liver tissue where it contributes to the formation of the C8 protein complex a component necessary for the assembly of the membrane attack complex (MAC). Other names for this target include complement component C8 beta chain emphasizing its role in the complement cascade.

Biological function summary

The C8B protein facilitates the stabilization of the C8 complex which also consists of the alpha and gamma chains to perform its role in the immune system. It plays an important role in forming the MAC an important structure for lysing pathogenic cells like bacteria by disrupting their plasma membranes. This action highlights the importance of C8B in innate immunity where it enables the host to eliminate harmful cells effectively.

Pathways

C8B's activities are integral to the complement cascade specifically within the terminal membrane attack pathway. It functions in conjunction with other complement factors like complement factor 5 to 9 forming a cascade of sequential activations. C8B works closely with complement factor 8 alpha and complement factor C5 within this pathway to achieve effective immune responses. These interactions ensure the proper function of the immune surveillance mechanism allowing the organism to protect itself against microbial infections.

C8B has significant connections to immunodeficiencies and inflammatory conditions. Patients with deficiencies in any of the components forming the MAC including C8B often suffer from recurrent Neisserial infections due to impaired bacterial lysis. C8B mutations contribute to properdin-independent pathway issues that can lead to susceptibility to such infections. Moreover malfunction in C8B can associate with autoimmune disorders implying a disruption in the delicate balance of the immune response where proteins like factor 8 and complement factor 5 play critical roles in maintaining immunity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 22832194, PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 7440581). The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways (PubMed : 30552328, PubMed : 39914456, PubMed : 39814882, PubMed : 7440581). The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 30552328, PubMed : 7440581). C8B, together with C8A and C8G, inserts into the target membrane, but does not form pores by itself (PubMed : 30552328). During MAC assembly, associates with C5b, C6 and C7 to form the C5b8 intermediate complex that inserts into the target membrane and traverses the bilayer increasing membrane rigidity (PubMed : 30552328, PubMed : 6833260).

See full target information C8B

대체 명칭 보기

Complement component C8 beta chain, Complement component 8 subunit beta, C8B

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

Immunoprecipitation - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (AB278049)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise