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AB316819

Anti-Complement Factor B (Ba fragment) antibody [EPR24245-52] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal CFAB antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-Complement Factor B (Ba fragment) antibody [EPR24245-52] - BSA and Azide free (Detector) (AB316819)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Complement Factor B (Ba fragment) antibody [EPR24245-52] - BSA and Azide free (Detector) (AB316819)

Sandwich ELISA of ab316817 with the capture antibody ab316818 dilution at 2 ug/mL and detector antibody ab316819 dilution at 0.5 ug/mL.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24245-52

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody has 100% cross reaction with full-length human Factor B.</p>

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보완 제품

Assay Kits

AB300318

Human Factor B ELISA Kit

Recombinant|SimpleStep

ELISA - Human Factor B ELISA Kit (AB300318)

  • 4
  • 1
Sample Prep & Detection Kits

AB102893

PE/Cy5® Conjugation Kit - Lightning-Link®

Flow Cytometry - PE/Cy5® Conjugation Kit - Lightning-Link® (AB102893)

  • 0
  • 0
Sample Prep & Detection Kits

AB154873

Gold Conjugation Kit (40nm, 20 OD)

Conjugation - Gold Conjugation Kit (40nm, 20 OD) (AB154873)

  • 0
  • 0
Sample Prep & Detection Kits

AB201795

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link®

ELISA - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (AB201795)

  • 0
  • 0
Proteins & Peptides

AB274645

Recombinant Human Factor B protein

SDS-PAGE - Recombinant Human Factor B protein (AB274645)

  • 0
  • 0
Primary Antibodies

AB90814

Anti-C3 antibody [bH6]

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab316819 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Complement Factor B (Ba fragment). The recommended pair for sandwich ELISA is:

Capture: ab316818, Human Complement Factor B (Ba fragment) Capture Antibody (unconjugated)

Detector: ab316819, Human Complement Factor B (Ba fragment) Detector Antibody (unconjugated)

The reference range value is 0.16-20 ng/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Complement Factor B also known as CFB Ba fragment or C3 proactivator participates mechanically in the alternative complement pathway. It has a molecular mass of approximately 93 kDa. Expressed mainly in the liver and found in plasma Factor B cleaves into two fragments Bb and Ba by Factor D. The Ba fragment specifically is known for its interactions that modulate complement activation. Ba itself although smaller is an active part of this cleavage event.
Biological function summary

Complement Factor B is important in the innate immune response. It forms a complex called the C3 convertase when combined with C3b and Bb fragment which amplifies the cascade leading to pathogen opsonization and lysis. The Ba fragment though not part of the convertase influences cell signaling and modulates inflammatory responses. It directly affects immune cells by binding to receptors and therefore impacts the regulation of immune responses.

Pathways

The alternative complement pathway and the regulation of inflammation fall under the influence of Complement Factor B. Within the complement pathway its interaction with proteins like C3 and Factor D emphasizes its position. This pathway is part of wider immune management processes highlighting its key role interacting with these proteins to control activation and amplification of the complement system leading to enhanced pathogen clearance and aiding the defense mechanism.

Complement Factor B shows connections to age-related macular degeneration (AMD) and atypical hemolytic uremic syndrome (aHUS). In AMD abnormal regulation of the complement pathway with involvement of Complement Factor B contributes to drusen formation complicating the disease progression. It also links to aHUS where uncontrolled complement activation causes endothelial damage. Proteins such as Complement Factor H which modulates the alternative pathway show a significant relationship with Factor B in these disease processes affecting the progression and control of these disorders through complement regulation.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). CFB is cleaved and activated by CFD to generate Ba and Bb chains; Bb chain constituting the catalytic component of the C3 and C5 convertases (PubMed : 6769474, PubMed : 9748277).. Complement factor B Bb. Serine protease component of the complement C3 and C5 convertase complexes of the alternative complement pathway (PubMed : 30643019, PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543). Following cleavage and activation by factor D (CFD), forms the C3 convertase together with complement C3b (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). As part of the C3 convertase, cleaves and activates C3 into C3a anaphylatoxin and C3b opsonin, the next components of the complement pathways (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). When an additional complement C3b molecule binds to the C3 convertase, forms the C5 convertase, which cleaves and activates C5 into C5a anaphylatoxin and C5b component of the membrane attack complex (PubMed : 30643019, PubMed : 624565, PubMed : 6554279).. Complement factor B Ba. Involved in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes.
See full target information CFB

대체 명칭 보기

BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG
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Product promise

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