AB129504

Anti-COMT antibody

Name: Anti-COMT antibody
Brand: Abcam Limited
SKU: AB129504
Rating: 5 (1 reviews)

(1 리뷰)

(3 제품이 사용된 논문 )

Rabbit Polyclonal COMT antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Catechol O-methyltransferase aa 200 to C-terminus.

대체 명칭 보기

Catechol O-methyltransferase, COMT

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody (AB129504)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody (AB129504)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling COMT with ab129504 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-COMT antibody (AB129504)

ab129504 at 1 μg/ml staining COMT by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab129504 at 6 μg/mg lysate.
Lane 2; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised Chemiluminescence with a 10 second exposure.

All lanes:

Immunoprecipitation - Anti-COMT antibody (ab129504)

Predicted band size: 30 kDa

false

Unknown

Western blot - Anti-COMT antibody (AB129504)

All lanes:

Western blot - Anti-COMT antibody (ab129504) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from 293T cells at 50 µg

Lane 4:

Whole cell lysate from Jurkat cells at 50 µg

Predicted band size: 30 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human Catechol O-methyltransferase aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P21964

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

COMT also known as catechol-O-methyltransferase is an enzyme responsible for the methylation of catechol compounds including neurotransmitters like dopamine epinephrine and norepinephrine. This reaction requires S-adenosylmethionine as a methyl donor. COMT primarily has two forms: a soluble form and a membrane-bound form with masses around 24 kDa and 30 kDa respectively. This protein shows significant expression in the liver brain and kidneys. COMT expression is also noted in cell lines such as HEK293T where it plays an important role in catecholamine metabolism.

Biological function summary

Catechol-O-methyltransferase serves as a regulator of neurotransmitter levels in the central nervous system and peripheral tissues. It does not appear to form part of a larger protein complex functioning mainly as a singular entity for metabolizing catechols. Through its action COMT impacts processes such as mood regulation cognition and stress response. Its role in degrading dopamine is especially significant in regions like the prefrontal cortex where dopamine plays an important role in executive functions.

Pathways

COMT participates in the catecholamine degradation pathway and impacts dopaminergic signaling. It works alongside enzymes such as monoamine oxidase (MAO) to regulate levels of neurotransmitters. COMT's activity influences the synaptic presence of dopamine and its interaction in signaling pathways. Moreover it indirectly affects the balance within the dopaminergic system which is important for normal neurological function and behavior.

COMT has links to psychiatric and neurological conditions such as schizophrenia and Parkinson’s disease. Variations in COMT function can affect dopamine metabolism which may contribute to the etiology of these disorders. Additionally proteins like MAO and dopamine transporter (DAT) also connect to COMT through their shared involvement in neurotransmitter regulation. Alterations in COMT activity can influence disease severity and patient response to treatment underlining its importance in understanding these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

See full target information Catechol O-methyltransferase

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 13:1285 PubMed36690660

2023

Catechol-O-methyl transferase suppresses cell invasion and interplays with MET signaling in estrogen dependent breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Janacova,Michaela Stenckova,Petr Lapcik,Sarka Hrachovinova,Pavla Bouchalova,David Potesil,Roman Hrstka,Petr Müller,Pavel Bouchal

Journal of environmental science and health. Part C, Toxicology and carcinogenesis 40:106-118 PubMed35895929

2022

The expression of Phase II drug-metabolizing enzymes in human B-lymphoblastoid TK6 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xilin Li,Yuxi Li,Kylie G Ning,Si Chen,Lei Guo,Jessica A Bonzo,Nan Mei

Cell death & disease 10:919 PubMed31801939

2019

Characterization of the role of TMEM45A in cancer cell sensitivity to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Kathleen Schmit,Jia-Wei Chen,Sophie Ayama-Canden,Maude Fransolet,Laure Finet,Catherine Demazy,Lionel D'Hondt,Carlos Graux,Carine Michiels

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COMT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody (AB129504)

Immunoprecipitation - Anti-COMT antibody (AB129504)

Western blot - Anti-COMT antibody (AB129504)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise