AB87645

Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Name: Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker
Brand: Abcam Limited
SKU: AB87645
Rating: 5 (2 reviews)

(2 리뷰들)

(19 제품이 사용된 논문 )

Goat Polyclonal Connexin 43 / GJA1 antibody. Suitable for WB and reacts with Rat samples. Cited in 19 publications.

1 이미지

Unknown

Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB87645)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab87645) at 0.3 µg/mL

All lanes:

rat brain lysate (in RIPA buffer) at 35 µg

Predicted band size: 43 kDa

Observed band size: 43 kDa

true

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab87645 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.

Biological function summary

This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.

Pathways

Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.

Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small molecules and electrical signals between neighboring cells (By similarity). Forms homotypic and heterotypic channels gated by transjunctional voltage (By similarity). May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph (Probable). Negative regulator of bladder functional capacity : acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in the conductive system of ventricular myocardium and heart morphogenesis (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization (By similarity). Involved in intercellular innate immune signaling (PubMed : 24077100, PubMed : 31992625, PubMed : 40010341). Mediates translocation of 2',3'-cGAMP and 2',5'-oligoadenylates (2-5A) second messengers from virus-infected cells to macrophages and uninfected neighboring cells to propagate and amplify the antiviral immune response (PubMed : 24077100, PubMed : 31992625, PubMed : 40010341).

See full target information GJA1

대체 명칭 보기

GJAL, GJA1, Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, Cx43

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제품이 사용된 논문 (19)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomolecules 14: PubMed39334940

2024

Connexin Expression Is Altered in the Eye Development of Mice: A Preliminary Study.

Applications

Unspecified application

Species

Unspecified reactive species

Ljubica Skelin,Anita Racetin,Nela Kelam,Marin Ogorevc,Ljubo Znaor,Mirna Saraga-Babić,Natalija Filipović,Yu Katsuyama,Zenon Pogorelić,Katarina Vukojević

Journal of molecular and cellular cardiology 179:7-17 PubMed36977444

2023

High-quality nuclei isolation from postmortem human heart muscle tissues for single-cell studies.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Araten,Ronald Mathieu,Anushka Jetly,Hoon Shin,Nazia Hilal,Bo Zhang,Katherine Morillo,Deepa Nandan,Indu Sivankutty,Ming Hui Chen,Sangita Choudhury

International journal of molecular sciences 23: PubMed35682927

2022

A Lack of GD3 Synthase Leads to Impaired Renal Expression of Connexins and Pannexin1 in Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Meter,Anita Racetin,Katarina Vukojević,Marta Balog,Vedrana Ivić,Milorad Zjalić,Marija Heffer,Natalija Filipović

International journal of molecular sciences 23: PubMed35682601

2022

The Expression of Connexin 37, 40, 43, 45 and Pannexin 1 in the Early Human Retina and Choroid Development and Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Matea Žužul,Mirela Lozić,Natalija Filipović,Samir Čanović,Ana Didović Pavičić,Joško Petričević,Nenad Kunac,Violeta Šoljić,Mirna Saraga-Babić,Suzana Konjevoda,Katarina Vukojevic

Biomedicines 10: PubMed35327391

2022

The Interplay of Cx26, Cx32, Cx37, Cx40, Cx43, Cx45, and Panx1 in Inner-Ear Development of Yotari (dab1-/-) Mice and Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Josip Lesko,Pejana Rastović,Josip Mišković,Violeta Šoljić,Vlatka Paštar,Zdenka Zovko,Natalija Filipović,Yu Katsuyama,Mirna Saraga-Babić,Katarina Vukojević

Life (Basel, Switzerland) 11: PubMed34947861

2021

Chronic Stress and Gonadectomy Affect the Expression of Cx37, Cx40 and Cx43 in the Spinal Cord.

Applications

Unspecified application

Species

Unspecified reactive species

Marija Jurić,Marta Balog,Vedrana Ivić,Benjamin Benzon,Anita Racetin,Ivana Bočina,Nives Kević,Suzana Konjevoda,Kálmán F Szűcs,Róbert Gáspár,Marija Heffer,Katarina Vukojević,Sandor G Vari,Natalija Filipović

International journal of molecular sciences 22: PubMed34639052

2021

Connexin Expression Is Altered in Liver Development of () Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Vlatka Paštar,Mirela Lozić,Nela Kelam,Natalija Filipović,Branka Bernard,Yu Katsuyama,Katarina Vukojević

Neural regeneration research 17:594-600 PubMed34380899

2021

The mechanisms through which auricular vagus nerve stimulation protects against cerebral ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Jun Zhao,Zheng-Hui Wang,Ying-Jie Zhang,Wen-Jing Wang,Ai-Fang Cheng,Pei-Jing Rong,Chun-Lei Shan

International journal of molecular sciences 22: PubMed33525532

2021

Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- () Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mirela Lozić,Natalija Filipović,Marija Jurić,Ivona Kosović,Benjamin Benzon,Ivana Šolić,Nela Kelam,Anita Racetin,Koichiro Watanabe,Yu Katsuyama,Masaki Ogata,Mirna Saraga-Babić,Katarina Vukojević

International journal of molecular sciences 21: PubMed33302507

2020

Expression of Connexins 37, 43 and 45 in Developing Human Spinal Cord and Ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Marija Jurić,Julia Zeitler,Katarina Vukojević,Ivana Bočina,Maximilian Grobe,Genia Kretzschmar,Mirna Saraga-Babić,Natalija Filipović

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB87645)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (19)

Product promise