AB235833

Anti-COTL1 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal COTL1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human COTL1.

대체 명칭 보기

CLP, COTL1, Coactosin-like protein

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COTL1 antibody (AB235833)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COTL1 antibody (AB235833)

Paraffin-embedded human lymphoid tissue stained for COTL1 using ab235833 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COTL1 antibody (AB235833)

Paraffin-embedded human lung cancer tissue stained for COTL1 using ab235833 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human COTL1.

Q14019

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

COTL1 also known as Coactosin-like protein is a small protein of approximately 16 kDa. This protein is expressed in a variety of tissues with notable presence in hematopoietic cells such as leukocytes. COTL1 binds actin and is involved mechanically in modulating actin polymerization. This protein also interacts with arachidonate 5-lipoxygenase (ALOX5) facilitating its enzymatic activity which plays an important role in lipid metabolism.

Biological function summary

COTL1 plays an important role in the regulation of immune responses and inflammatory processes. It acts as a cofactor stabilizing the interaction between ALOX5 and actin filaments impacting the formation of leukotrienes which mediate immune responses. COTL1 is not known to be part of a larger protein complex but it influences the function of ALOX5 suggesting a collaborative role in cellular processes related to inflammation.

Pathways

COTL1 influences the biosynthesis of leukotrienes within the arachidonic acid metabolic pathway. This pathway is significant for inflammatory response modulation. COTL1 and ALOX5 together affect the production of leukotrienes providing a connection to the eicosanoid signaling pathway. This involvement positions COTL1 as an important regulator of inflammation.

COTL1 has connections with inflammatory disorders and certain cancers. The protein's interaction with ALOX5 links it to conditions such as asthma where leukotriene pathways play a part in pathogenesis. Overexpression or dysregulation of COTL1 can also relate to specific cancers including leukemia where inflammatory signaling contributes to disease progression. Understanding the COTL1-protein interactions may offer therapeutic potential.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis.

See full target information COTL1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of translational medicine 21:53 PubMed36709284

2023

Upregulation of HMGB1 in tumor-associated macrophages induced by tumor cell-derived lactate further promotes colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Gao,Shiqi Zhou,Zhaofu Qin,Dechuan Li,Yuping Zhu,Dening Ma

Cancer management and research 12:10909-10917 PubMed33154670

2020

Coactosin-Like Protein (COTL1) Promotes Glioblastoma (GBM) Growth in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Shike Shao,Yongjun Fan,Chongpei Zhong,Xianlong Zhu,Jiaqiu Zhu

Gland surgery 9:747-758 PubMed32775265

2020

Downregulation of microRNA-30c-5p was responsible for cell migration and tumor metastasis via COTL1-mediated microfilament arrangement in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Pei,Tongyang Li,Qi Qian,Wenqiang Fan,Xiao He,Yulan Zhu,Lingyun Xu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COTL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COTL1 antibody (AB235833)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COTL1 antibody (AB235833)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise