AB190251

Anti-COX16 antibody

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Rabbit Polyclonal COX16 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COX16 aa 50 to C-terminus.

대체 명칭 보기

C14orf112, HSPC203, PTD019, COX16, hCOX16

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX16 antibody (AB190251)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX16 antibody (AB190251)

Immunohistochemical analysis of paraffin-embedded human smooth muscle tissue labeling COX16 in the cytoplasm and membrane of smooth muscle cells with ab190251 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-COX16 antibody (AB190251)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX16 antibody (AB190251)

Immunocytochemical analysis of MCF7 (Human breast adenocarcinoma cell line) whole cells labeling COX16 in the mitochondria with ab190251 at 2 μg/ml.

Supplier Data

Western blot - Anti-COX16 antibody (AB190251)

All lanes:

Western blot - Anti-COX16 antibody (ab190251) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate

Predicted band size: 12 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human COX16 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P0S2

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

COX16 also known as cytochrome c oxidase assembly factor COX16 is a mitochondrial membrane protein with a molecular mass of approximately 16 kDa. This protein is expressed primarily in tissues with high energy demands such as skeletal muscles heart and brain. It resides in the inner mitochondrial membrane where it assists in the stabilization and assembly of cytochrome c oxidase an important component of the mitochondrial respiratory chain.

Biological function summary

COX16 plays a role in facilitating the assembly of cytochrome c oxidase which is a multi-subunit complex. This enzyme complex is responsible for the final step in the electron transport chain a process critical for ATP production in cells. COX16 contributes to the incorporation of subunit Cox1 into the growing enzyme complex ensuring proper enzyme functionality and cellular energy production.

Pathways

COX16 is integral to mitochondrial oxidative phosphorylation and respiratory chain complexes formation. It interacts closely with other proteins such as SURF1 and LRPPRC which are also involved in cytochrome c oxidase assembly. Dysfunction in these pathways can lead to impaired energy production and metabolic disturbances at the cellular level.

COX16 mutations have been linked to disorders like mitochondrial complex IV deficiency and Leigh syndrome. These diseases manifest with neurological and muscular symptoms due to impaired energy metabolism. COX16's dysfunction in these conditions often involves interactions with proteins like COX10 and COX2 further implicating the cytochrome oxidase complex's role in disease pathology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase (PubMed : 29355485, PubMed : 29381136, PubMed : 33169484). Promotes the insertion of copper into the active site of cytochrome c oxidase subunit II (MT-CO2/COX2) (PubMed : 29355485, PubMed : 29381136). Interacts specifically with newly synthesized MT-CO2/COX and its copper center-forming metallochaperones SCO1, SCO2 and COA6 (PubMed : 29381136). Probably facilitates MT-CO2/COX2 association with the MITRAC assembly intermediate containing MT-CO1/COX1, thereby participating in merging the MT-CO1/COX1 and MT-CO2/COX2 assembly lines (PubMed : 29381136).

See full target information COX16

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Hereditas 161:27 PubMed39192374

2024

The novel circFKBP8/miR-432-5p/E2F7 cascade functions as a regulatory network in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongkui Jin,Wang Xu,Kunlin Yu,Cailu Luo,Xiaodan Luo,Tao Lian,Changshan Liu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COX16 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX16 antibody (AB190251)

Immunocytochemistry/ Immunofluorescence - Anti-COX16 antibody (AB190251)

Western blot - Anti-COX16 antibody (AB190251)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise