AB69611

Anti-COX17 antibody

Name: Anti-COX17 antibody
Brand: Abcam Limited
SKU: AB69611
Rating: 1 (1 reviews)

(1 리뷰)

(6 제품이 사용된 논문 )

Rabbit Polyclonal COX17 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human COX17.

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX17 antibody (AB69611)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX17 antibody (AB69611)

ab69611, at 1/50 dilution, staining human liver carcinoma tissue in the presence or absence of the immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-COX17 antibody (AB69611)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-COX17 antibody (AB69611)

ICC/IF image of ab69611 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69611, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-COX17 antibody (AB69611)

All lanes:

Western blot - Anti-COX17 antibody (ab69611) at 1/500 dilution

Lane 1:

Extracts from HeLa cells at 5 µg

Lane 2:

Extracts from HepG2 cells at 5 µg

Lane 3:

Extracts from COLO cells at 5 µg

Lane 4:

Extracts from 293 cells at 5 µg

Lane 5:

Extracts from COLO cells at 5 µg with immunising peptide

Predicted band size: 6 kDa

Observed band size: <19 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human COX17. The exact immunogen used to generate this antibody is proprietary information.

Q14061

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

COX17 also known as Cytochrome c oxidase copper chaperone plays a mechanical role by providing copper ions to cytochrome c oxidase in mitochondria. It weighs approximately 8 kDa and is expressed in various tissues with a higher presence in heart and skeletal muscle tissues. This small protein ensures the proper functioning of cytochrome c oxidase through copper ion delivery an essential process for mitochondrial energy production.

Biological function summary

COX17 assists in the formation and stabilization of the cytochrome c oxidase complex a critical component of the mitochondrial electron transport chain. As part of this complex COX17 ensures efficient electron transfer and ATP production by donating copper ions to support the catalytic activity. Proper cytochrome c oxidase function maintains cellular respiration and energy synthesis which is necessary for cellular vitality and health.

Pathways

COX17 is involved in the mitochondrial electron transport chain and copper ion homeostasis pathways. These pathways are vital for maintaining cellular energy levels and regulating copper distribution within cells. COX17 interacts with other proteins such as COX11 and COX17 plays an essential supportive role alongside COX11 ensuring cytochrome c oxidase assembles correctly and functioning optimally to utilize oxygen effectively.

COX17 has a connection with mitochondrial disorders and neurodegenerative conditions. Mutations or dysfunctions in COX17 can lead to deficits in cellular energy production contributing to conditions like Leigh syndrome and some forms of muscular dystrophy. Its interaction with cytochrome c oxidase makes abnormalities in COX17 relevant to cytochrome c oxidase deficiency implicating it in diseases influenced by mitochondrial malfunction.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Copper metallochaperone essential for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. Binds two copper ions and delivers them to the metallochaperone SCO1 which transports the copper ions to the Cu(A) site on the cytochrome c oxidase subunit II (MT-CO2/COX2).

See full target information COX17

대체 명칭 보기

Cytochrome c oxidase copper chaperone, COX17

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 15:3246 PubMed38622137

2024

Alkyne-tagged SERS nanoprobe for understanding Cu and Cu conversion in cuproptosis processes.

Applications

Unspecified application

Species

Unspecified reactive species

Sihan Zhang,Yuxiao Mei,Jiaqi Liu,Zhichao Liu,Yang Tian

Molecular nutrition & food research 65:e2001202 PubMed34075698

2021

Diosgenin Protects Against Kidney Injury and Mitochondrial Apoptosis Induced by 3-MCPD Through the Regulation of ER Stress, Ca Homeostasis, and Bcl2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Zhong,Chengni Jin,Jiahui Han,Jiachang Zhu,Qi Liu,Dianjun Sun,Xiaodong Xia,Yu Zhang,Xiaoli Peng

Biomedicines 8: PubMed33287280

2020

The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Jake J Wen,Claire B Cummins,Taylor P Williams,Ravi S Radhakrishnan

Scientific reports 10:3571 PubMed32107448

2020

Endothelin-1 Mediated Decrease in Mitochondrial Gene Expression and Bioenergetics Contribute to Neurodegeneration of Retinal Ganglion Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Renuka M Chaphalkar,Dorota L Stankowska,Shaoqing He,Bindu Kodati,Nicole Phillips,Jude Prah,Shaohua Yang,Raghu R Krishnamoorthy

Chemico-biological interactions 296:9-17 PubMed30107153

2018

Protective effects of apigenin against 3-MCPD-induced renal injury in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Zhong,Chengni Jin,Xiaorui Wang,Xuan Li,Jiahui Han,Wei Xue,Peng Wu,Xiaoli Peng,Xiaodong Xia

Biochimica et biophysica acta 1863:198-204 PubMed26589182

2015

Direct structural evidence of protein redox regulation obtained by in-cell NMR.

Applications

Unspecified application

Species

Unspecified reactive species

Eleonora Mercatelli,Letizia Barbieri,Enrico Luchinat,Lucia Banci

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COX17 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX17 antibody (AB69611)

Immunocytochemistry/ Immunofluorescence - Anti-COX17 antibody (AB69611)

Western blot - Anti-COX17 antibody (AB69611)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise