JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB70112

Anti-COX4I2 antibody

Be the first to review this product! 리뷰 제출

|

(7 제품이 사용된 논문 )

Rabbit Polyclonal COX42 antibody. Suitable for WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human COX4I2 aa 1-100.
2 이미지
Western blot - Anti-COX4I2 antibody (AB70112)
  • WB

Unknown

Western blot - Anti-COX4I2 antibody (AB70112)

All lanes:

Western blot - Anti-COX4I2 antibody (ab70112) at 1/500 dilution

Lane 1:

K562 cell extracts treated with insulin (0.01U/ml, 15mins) at 30 µg

Lane 2:

K562 cell extracts treated with insulin (0.01U/ml, 15mins) at 30 µg with immunising peptide

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-COX4I2 antibody (AB70112)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-COX4I2 antibody (AB70112)

ab70112 at 1/500 dilution staining COX4I2 in COS7 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, African green monkey

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human COX4I2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q96KJ9

style
left-column

보완 제품

Primary Antibodies

AB110261

Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker

Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (AB110261)

  • 3
  • 2
Primary Antibodies

AB181125

Anti-STING antibody [EPR13130]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-STING antibody [EPR13130] (AB181125)

  • 4
  • 1
Primary Antibodies

AB184787

Anti-Caspase-3 antibody [EPR18297]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [EPR18297] (AB184787)

  • 3
  • 3
Primary Antibodies

AB76493

Anti-IRF3 (phospho S386) antibody [EPR2346]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (AB76493)

  • 4
  • 2
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab70112 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The COX4I2 also known as cytochrome c oxidase subunit 4 isoform 2 is a protein that plays an important role in the electron transport chain in mitochondria. It has a molecular mass of approximately 19 kDa. COX4I2 is expressed in mitochondrial membranes and is particularly found in tissues with high oxygen demand like the heart and skeletal muscles. Unlike its close relative COX4I1 COX4I2 is expressed variably depending on the oxygen availability in the tissues where it functions.
Biological function summary

COX4I2 contributes to cellular energy production by being part of the cytochrome c oxidase complex also known as complex IV of the electron transport chain. This complex is central in the final step of electron transfer during cellular respiration facilitating the reduction of oxygen to water. By doing so it aids in establishing the proton gradient that drives ATP synthesis. Its expression adjusts according to oxygen levels which suggests a responsive role in adapting cellular metabolism to hypoxic conditions.

Pathways

COX4I2 is integrally involved in the oxidative phosphorylation pathway. In this pathway it collaborates with other complex IV subunits to influence the respiratory chain efficiency and energy production. COX4I2 works alongside other proteins like COX1 and COX2 ensuring effective electron transfer and proton pumping. Additionally it has connections to the hypoxia-inducible factor (HIF) pathway influencing how cells respond to low oxygen conditions.

COX4I2 has links to conditions like cancer and mitochondrial disorders. Hypoxia a common feature in tumor microenvironments often leads to increased COX4I2 expression adapting the cancer cell metabolism to survive low oxygen. In mitochondrial disorders improper function or regulation of COX4I2 can impair ATP production leading to symptoms in high energy-demand tissue. COX4I2 might interact with other mitochondrial proteins such as COX4I1 in these scenarios affecting the overall function of the respiratory chain in pathological states.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
See full target information COX4I2

대체 명칭 보기

COX4L2, COX4I2, Cytochrome c oxidase subunit IV isoform 2, COX IV-2
style
left-column

제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 25:1786-1799.e4 PubMed30428348

2018

Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Lobo-Jarne,Eva Nývltová,Rafael Pérez-Pérez,Alba Timón-Gómez,Thibaut Molinié,Austin Choi,Arnaud Mourier,Flavia Fontanesi,Cristina Ugalde,Antoni Barrientos

The Journal of biological chemistry 293:3637-3650 PubMed29343514

2018

TRPM2 channel-mediated regulation of autophagy maintains mitochondrial function and promotes gastric cancer cell survival via the JNK-signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shekoufeh Almasi,Barry E Kennedy,Mariam El-Aghil,Andra M Sterea,Shashi Gujar,Santiago Partida-Sánchez,Yassine El Hiani

Bioscience reports 37: PubMed29026004

2017

Oxidative stress regulates cellular bioenergetics in esophageal squamous cell carcinoma cell.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Zhang,Linhua Lan,Lili Niu,Juping Lu,Changxi Li,Miaomiao Guo,Shouyong Mo,Jing Lu,Yongzhang Liu,Bin Lu

EMBO reports 18:477-494 PubMed28082314

2017

A -knockout reveals translation-independent control of human mitochondrial complex IV biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Myriam Bourens,Antoni Barrientos

PloS one 9:e104971 PubMed25127027

2014

Loss of Intralipid®- but not sevoflurane-mediated cardioprotection in early type-2 diabetic hearts of fructose-fed rats: importance of ROS signaling.

Applications

WB

Species

Rat

Phing-How Lou,Eliana Lucchinetti,Liyan Zhang,Andreas Affolter,Manoj Gandhi,Martin Hersberger,Blair E Warren,Hélène Lemieux,Hany F Sobhi,Alexander S Clanachan,Michael Zaugg

The Journal of cell biology 187:959-66 PubMed20038677

2009

Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Brian Head,Lorena Griparic,Mandana Amiri,Shilpa Gandre-Babbe,Alexander M van der Bliek

Proceedings of the National Academy of Sciences of 105:14447-52 PubMed18794531

2008

A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis.

Applications

WB

Species

Mouse

Bong-Hyun Ahn,Hyun-Seok Kim,Shiwei Song,In Hye Lee,Jie Liu,Athanassios Vassilopoulos,Chu-Xia Deng,Toren Finkel
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com