JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248913

Anti-COX7B antibody [EPR9326(B)] - BSA and Azide free

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Recombinant Monoclonal COX7B antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

Cytochrome c oxidase polypeptide VIIb, COX7B

1 이미지
Western blot - Anti-COX7B antibody [EPR9326(B)] - BSA and Azide free (AB248913)
  • WB

Lab

Western blot - Anti-COX7B antibody [EPR9326(B)] - BSA and Azide free (AB248913)

This data was developed using the same antibody clone in a different buffer formulation (ab140629).

Lanes 1-3 : Merged signal (red and green). Green - ab140629 observed at 13 kDa. Red - loading control ab8245 observed at 36 kDa.

ab140629 Anti-COX7B antibody [EPR9326(B)] was shown to specifically react with COX7B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265549 (knockout cell lysate ab257897) was used. Wild-type and COX7B knockout samples were subjected to SDS-PAGE. ab140629 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-COX7B antibody [EPR9326(B)] (<a href='/ko/products/primary-antibodies/cox7b-antibody-epr9326b-ab140629'>ab140629</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

COX7B knockout HeLa cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 9 kDa

Observed band size: 13 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9326(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Primary Antibodies

AB150422

Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

  • 0
  • 0
Primary Antibodies

AB203912

Anti-MTCO1 antibody [EPR19628]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] (AB203912)

  • 0
  • 0
Reagents, Controls & Accessories

AB110413

Total OXPHOS Rodent WB Antibody Cocktail

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

  • 5
  • 18
Primary Antibodies

AB129752

Anti-NDUFA4 antibody

Western blot - Anti-NDUFA4 antibody (AB129752)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

제품 세부 정보

ab248913 is the carrier-free version of ab140629.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

COX7B also known as Cytochrome c oxidase subunit 7B is a small protein with a molecular mass of approximately 11 kDa. It performs a mechanical function as a component of the cytochrome c oxidase (COX) complex which is the terminal enzyme of the mitochondrial electron transport chain. COX7B is widely expressed in tissues with high energy demand such as the heart muscle and brain. The protein enables the electron transport chain to conduct its role efficiently by aiding the enzyme's proper assembly and stability.
Biological function summary

Within the architecture of aerobic respiration COX7B functions as a vital component of the protein complex cytochrome c oxidase also referred to as Complex IV. This complex is important for the last step of the electron transport chain facilitating the transfer of electrons from cytochrome c to molecular oxygen. During this process COX7B participates in promoting the creation of a proton gradient across the mitochondrial inner membrane which is an important step in ATP synthesis.

Pathways

Multiple cellular energy production pathways include COX7B's activity such as oxidative phosphorylation and the mitochondrial respiratory chain pathway. It interacts with proteins like cytochrome c and other subunits within Complex IV to ensure effective cellular respiration. By acting in the electron transport chain COX7B helps in controlling the flow of electrons and maintaining mitochondrial function which is essential for metabolic balance.

Mitochondrial dysfunctions involving COX7B have associations with particular conditions like Leigh syndrome and mitochondrial complex IV deficiency. These disorders connect COX7B to the pathology where energy production becomes compromised. Other proteins such as those in the COX complex and components of the mitochondrial maze link indirectly with COX7B contributing to disease understanding due to their shared involvement in respiratory defects.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix (By similarity). Plays a role in proper central nervous system (CNS) development in vertebrates (PubMed : 23122588).
See full target information Cytochrome c oxidase subunit 7B, mitochondrial
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com