AB236606

Anti-CPNE3 antibody

Be the first to review this product! 리뷰 제출

(3 제품이 사용된 논문 )

Rabbit Polyclonal CPNE3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CPNE3 aa 250-450.

대체 명칭 보기

CPN3, KIAA0636, CPNE3, Copine-3, Copine III

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE3 antibody (AB236606)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE3 antibody (AB236606)

Paraffin-embedded human small intestine tissue stained for CPNE3 using ab236606 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE3 antibody (AB236606)

Paraffin-embedded human liver tissue stained for CPNE3 using ab236606 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CPNE3 antibody (AB236606)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CPNE3 antibody (AB236606)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CPNE3 (green) using ab236606 at 1/66 dilution in ICC/IF. Secondary antibody is Alexa Fluor^® 488-congugated Goat Anti-Rabbit IgG(H+L).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CPNE3 aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

O75131

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CPNE3 also known as Copine-3 is a calcium-dependent membrane-binding protein. This protein has a molecular mass of approximately 58 kDa and belongs to the copine family. It consists of two common elements the C2 domain and the A domain known as Von Willebrand factor type A (VWA). CPNE3 expresses in tissues like brain heart and skeletal muscle. These domains enable CPNE3 to associate with cellular membranes in response to calcium signaling implying a role in intracellular signal transduction.

Biological function summary

CPNE3 influences cellular processes such as membrane trafficking signal transduction and cytoskeletal organization. It is not currently known to be a part of any complex but it is thought to mediate interaction between various cellular components through its modular domains. Its role in signal transduction links it to processes like cell movement apoptosis and differentiation. CPNE3 potentially triggers cellular responses by interacting with other proteins and lipids within the cell environment.

Pathways

CPNE3 actively participates in signaling related to calcium influx and integrin pathways. It is associated with the calcium signaling pathway that is significant for various cellular activities like muscle contraction and neurotransmitter release. Additionally CPNE3's interaction within the integrin signaling pathway implicates its role in extracellular matrix adhesion and cellular assembly. Through these pathways CPNE3 interacts with proteins such as integrins and calmodulin influencing cell adhesion and motility.

CPNE3 relates closely to cancer and cardiovascular diseases. Its increased expression in some cancers suggests a connection to tumor progression and metastasis particularly in ovarian cancer and glioblastoma. CPNE3 is connected to proteins such as EGFR and Akt in the context of cancer which are key players in cell proliferation and survival pathways. In cardiovascular diseases its capability to influence muscle function relates to proteins involved in calcium homeostasis impacting conditions like hypertrophy.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Calcium-dependent phospholipid-binding protein that plays a role in ERBB2-mediated tumor cell migration in response to growth factor heregulin stimulation (PubMed : 20010870).

See full target information CPNE3

style

left-column

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 24: PubMed37373386

2023

Hif1α/Dhrs3a Pathway Participates in Lipid Droplet Accumulation via Retinol and Ppar-γ in Fish Hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Tian,Yihui Du,Binbin Wang,Mengmeng Ji,Hongyan Li,Yun Xia,Kai Zhang,Zhifei Li,Wenping Xie,Wangbao Gong,Ermeng Yu,Guangjun Wang,Jun Xie

Experimental and therapeutic medicine 23:128 PubMed34970351

2022

CPNE3 interaction with RACK1 protects against myocardial ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqun Zhang,Xue Han,Yanan Zhang

Journal of Cancer 12:7277-7286 PubMed35003348

2021

CPNE3 regulates the cell proliferation and apoptosis in human Glioblastoma via the activation of PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dainan Zhang,Xiaoyin Wang,Xi Wang,Zemin Wang,Shunchang Ma,Chuanbao Zhang,Shaomin Li,Wang Jia

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CPNE3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE3 antibody (AB236606)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE3 antibody (AB236606)

Immunocytochemistry/ Immunofluorescence - Anti-CPNE3 antibody (AB236606)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise