AB248388

Anti-CPSF73 antibody [EPR7468] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CPSF73 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

대체 명칭 보기

CPSF73, CPSF3, Cleavage and polyadenylation specificity factor subunit 3, Cleavage and polyadenylation specificity factor 73 kDa subunit, mRNA 3'-end-processing endonuclease CPSF-73, CPSF 73 kDa subunit

4 이미지

Flow Cytometry (Intracellular) - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388)

This data was developed using ab131245, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPSF73 (red) with ab131245 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388)

This data was developed using ab131245, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labelling CPSF73 with ab131245 at 1/50 dilution.

Unknown

Western blot - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388)

This data was developed using ab131245, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CPSF73 antibody [EPR7468] (<a href='/ko/products/primary-antibodies/cpsf73-antibody-epr7468-ab131245'>ab131245</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Lane 6:

PC-12 cell lysate at 10 µg

Lane 7:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 77 kDa

false

OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7468

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab248388 is the carrier-free version of ab131245.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CPSF73 also known as Cleavage and Polyadenylation Specificity Factor 73 is an important component of the mRNA processing machinery. Mechanically CPSF73 functions as an endonuclease that cleaves pre-mRNA at the 3' end a necessary step for the addition of a poly(A) tail. CPSF73 has a molecular mass of approximately 73 kDa. This protein is expressed ubiquitously across various cell types reflecting its essential role in gene expression regulation.

Biological function summary

The mRNA processing involves CPSF73 as part of the larger cleavage and polyadenylation complex. This complex is responsible for recognizing specific cleavage sites within pre-mRNA and is critical for mRNA maturation. CPSF73’s activity ensures the proper maturation of messenger RNA facilitating its export from the nucleus translation into proteins and subsequent degradation. The endonuclease works closely with other components of the complex including CPSF160 CPSF100 and CFIm suggesting that its role extends beyond just cleavage to broader mRNA stability and regulation.

Pathways

CPSF73 actively participates in the mRNA processing pathway playing an integral role in post-transcriptional regulation. It also interfaces with the spliceosome machinery illustrating the crosstalk between pre-mRNA splicing and polyadenylation sites. CPSF73 is closely related to other RNA processing proteins such as CstF-64 and Symplekin which together maintain the precise regulation of gene expression. This interrelation is particularly important in pathways where timing of mRNA processing impacts cellular function and response.

CPSF73's malfunction could lead to serious implications including cancer and genetic disorders. Aberrant expression or mutations in CPSF73 have been linked to various cancers as they directly affect mRNA maturation and stability which could result in unchecked cell proliferation. Furthermore its connection with proteins such as CPSF160 has been observed in cancers where dysregulation of mRNA processing pathways contribute to tumorigenesis. Disorders like these highlight the necessity of CPSF73 for maintaining cellular health and proper gene expression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as an mRNA 3'-end-processing endonuclease (PubMed : 30507380). Also involved in the histone 3'-end pre-mRNA processing (PubMed : 30507380). U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for entering/progressing through S-phase of the cell cycle (PubMed : 30507380). Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1 (By similarity). Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By similarity). Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (By similarity).

See full target information CPSF3

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CPSF73 antibody [EPR7468] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388)

Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388)

Western blot - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388)

OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise