AB47373

Anti-CREB (phospho S129) antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal CREB phospho S129 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CREB1 pS129 aa 50-150.

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB (phospho S129) antibody (AB47373)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB (phospho S129) antibody (AB47373)

Immunohistochemistry (Paraffin-embedded sections) using human breast carcinoma tissue and ab47373 at a dilution of 1/50.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

Immunogen

Synthetic Peptide within Human CREB1 pS129 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB194313

HRP Anti-CREB antibody [E306]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CREB antibody [E306] (AB194313)

0
0

Primary Antibodies

AB32096

Anti-CREB (phospho S133) antibody [E113]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S133) antibody [E113] (AB32096)

4
14

Primary Antibodies

AB220798

Anti-CREB (phospho S133) antibody [E113] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S133) antibody [E113] - BSA and Azide free (AB220798)

0
0

Primary Antibodies

AB32515

Anti-CREB antibody [E306]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [E306] (AB32515)

5
4

Primary Antibodies

AB221216

Anti-CREB antibody [E306] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [E306] - BSA and Azide free (AB221216)

0
0

보완 제품

Primary Antibodies

AB58528

Anti-Lamin A + Lamin C (phospho S392) antibody

Immunocytochemistry - Anti-Lamin A + Lamin C (phospho S392) antibody (AB58528)

4
1

Primary Antibodies

AB32096

Anti-CREB (phospho S133) antibody [E113]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S133) antibody [E113] (AB32096)

4
14

Primary Antibodies

AB32515

Anti-CREB antibody [E306]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [E306] (AB32515)

5
4

Primary Antibodies

AB138683

Anti-Topoisomerase II alpha (phospho S1525) antibody

Western blot - Anti-Topoisomerase II alpha (phospho S1525) antibody (AB138683)

0
0

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CAMP response element-binding protein better known as CREB functions as a transcription factor that regulates gene expression. CREB protein operates by binding to specific DNA sequences called cAMP response elements in the promoters of target genes leading to increased or altered transcription. The protein has a molecular weight around 43 kDa. Expressed broadly in many tissues CREB's activity is particularly high in the brain where it plays key roles in neuronal functions. Researchers also refer to phospho-CREB when discussing the activated form of this protein often analyzed through methods like CREB Western blot.

Biological function summary

CREB contributes significantly to cellular processes such as proliferation differentiation and survival through transcriptional regulation. CREB does not function alone; it frequently associates with other proteins forming complexes to exert its regulatory roles. Additionally CREB influences the development of memory and learning in neurons highlighting its extensive involvement in neurophysiological processes.

Pathways

CREB plays a substantial role in the cAMP signaling pathway as well as the mitogen-activated protein kinase (MAPK) pathway. Through its interaction with kinases and other signaling proteins CREB influences numerous downstream targets affecting cellular responses to external stimuli. Notably protein kinase A (PKA) activates CREB by phosphorylation facilitating its role in various signal transduction pathways and linking it to downstream transcriptional events.

Research links CREB to neurological disorders and some forms of cancer. Aberrant CREB activity associates with neurodegenerative diseases like Alzheimer’s where dysregulated gene expression impacts neuronal viability and function. Furthermore in cancer CREB's role in cell proliferation and survival ties it to oncogenes such as BCL2 where abnormal CREB function can promote tumorigenesis. Understanding these connections provides valuable insights for therapeutic strategies against such diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed : 14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).

See full target information CREB1 pS129

대체 명칭 보기

Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Progress in neuro-psychopharmacology & biological 95:109682 PubMed31265863

2019

Increased Homer1-mGluR5 mediates chronic stress-induced depressive-like behaviors and glutamatergic dysregulation via activation of PERK-eIF2α.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Xing Li,Qian Li,Xue-Jiao Sun,Can Luo,Yong Li,Ya-Nan Wang,Jing Chen,Chen-Zi Gong,Ya-Jie Li,Li-Ping Shi,Yi-Feng Zheng,Rong-Chun Li,Xiao-Lin Huang,Qiu-Ju Xiong,Hong Chen

Brain and behavior 5:e00359 PubMed26664784

2015

Neuritic complexity of hippocampal neurons depends on WIP-mediated mTORC1 and Abl family kinases activities.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Franco-Villanueva,Francisco Wandosell,Inés M Antón

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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