AB277098

Anti-CREB5 antibody [PCRP-CREB5-1G8]

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Mouse Monoclonal CREB5 antibody. Suitable for ICC/IF, Flow Cyt, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CREB5.

대체 명칭 보기

CREBPA, CREB5, Cyclic AMP-responsive element-binding protein 5, CREB-5, cAMP-responsive element-binding protein 5, cAMP-response element-binding protein A, CRE-BPa

5 이미지

Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Flow cytometry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CREB5 using ab277098 at 2 μg / 10⁶ cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunocytochemistry/ Immunofluorescence - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Immunofluorescence analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CREB5 with ab277098 at 2 μg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CREB5 using ab277098 at 2 μg / 10⁶ cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Supplier Data

Western blot - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

All lanes:

Western blot - Anti-CREB5 antibody [PCRP-CREB5-1G8] (ab277098) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 56 kDa

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Protein Array - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Protein Array

Supplier Data

Protein Array - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277098.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

PCRP-CREB5-1G8

Isotype

IgG2b

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, Protein Array, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CREB5.

Q02930

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from bioreactor concentrate

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CREB5 also known as cAMP responsive element binding protein 5 is a transcription factor important for regulating gene expression. This protein has a mass of approximately 57 kDa and belongs to the CREB family of proteins. It is found expressed in various tissues including the brain lung and kidney. CREB5 binds to the cAMP response element (CRE) sequence found in the promoters of certain genes to modulate their transcription.

Biological function summary

CREB5 plays a role in the response to cellular stress and adaptation. It participates in mediating cellular responses through transcriptional activation influenced by signals such as hormones growth factors and neurotransmitters. CREB5 forms complexes with other transcription factors and coactivators enhancing or repressing the transcription of target genes depending on the context. It influences processes such as differentiation proliferation and survival of cells.

Pathways

CREB5 is involved in the cAMP signaling pathways and MAPK/ERK pathways. These pathways regulate various cellular functions including metabolism cell cycle progression and synaptic plasticity. CREB5 interacts with proteins like CREB1 and CREB3 which together contribute to the dysregulation of these pathways when mutations or malfunctions occur.

CREB5 has been associated with neurodegenerative disorders like Alzheimer's disease and certain types of cancer such as lung cancer. Variants in the expression or function of CREB5 can markedly affect the disease process often interacting with proteins like ATF4 in Alzheimer's which is implicated in stress responses and with NF-kB in cancer involved in immune response and cell survival mechanisms. Understanding CREB5's role provides insights into therapeutic targets for these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds to the cAMP response element and activates transcription.

See full target information CREB5

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of gastrointestinal oncology 11:1186-1199 PubMed33456992

2021

CircBACH1/let-7a-5p axis enhances the proliferation and metastasis of colorectal cancer by upregulating CREB5 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jutang Li,Qian Tang,Wei Dong,Yizhou Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CREB5 antibody [PCRP-CREB5-1G8]

Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Immunocytochemistry/ Immunofluorescence - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Western blot - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Protein Array - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise