AB211438

Anti-CRLF1 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal CRLF1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CRLF1 aa 100 to C-terminus.

대체 명칭 보기

UNQ288/PRO327, CRLF1, Cytokine receptor-like factor 1, Cytokine-like factor 1, ZcytoR5, CLF-1

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRLF1 antibody (AB211438)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRLF1 antibody (AB211438)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human skeletal muscle tissue labeling CRLF1 with ab211438 at 7.5 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRLF1 antibody (AB211438)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human heart tissue labeling CRLF1 with ab211438 at 7.5 μg/ml.

Supplier Data

Western blot - Anti-CRLF1 antibody (AB211438)

10% SDS PAGE

All lanes:

Western blot - Anti-CRLF1 antibody (ab211438) at 1/3000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 46 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CRLF1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75462

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "7.5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 89% Tris glycine, 10% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CRLF1 also known as cytokine receptor-like factor 1 is a secreted protein with a molecular mass around 50 kDa. CRLF1 mainly expresses in the central nervous system and various other tissues including muscle and liver. It plays a role in immune function and neurodevelopment by forming part of a receptor complex. This protein assists in transmitting signals across cell membranes which influence growth differentiation and survival of certain cell types.

Biological function summary

Through forming a functional receptor complex with the cytokine cardiotrophin-like cytokine factor 1 (CLCF1) CRLF1 participates in cytokine signaling. The CRLF1-CLCF1 complex binds to a receptor composed of ciliary neurotrophic factor receptor (CNTFR) and leukemia inhibitory factor receptor (LIFR) activating intracellular pathways. This mechanism affects neuroprotective neurotrophic and immune-modulatory processes by promoting neuron survival and modulating immune responses.

Pathways

Its involvement in the JAK-STAT signaling pathway highlights CRLF1's role in modulating gene expression. The interactions with CRLF1 and LIFR are critical within this pathway influencing STAT3 phosphorylation and eventual transcriptional regulation. Though mainly implicated in the JAK-STAT pathway CRLF1 also overlaps with neurotrophic signaling processes working alongside proteins like CNTFR to mediate neural repair and precision immune regulation.

CRLF1 mutations are linked to several conditions including Cold-induced sweating syndrome and Crisponi syndrome—both related to dysregulation in neural and thermogenic processes. These syndromes often involve the CRLF1-associated pathway's failures to properly signal often resulting in issues consistent with complex disorders. Linked to LIFR through these pathologies CRLF1's malfunction significantly contributes to disrupted signaling networks emphasizing its association with specific developmental and immune malfunctions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

In complex with CLCF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development (Probable). May also play a regulatory role in the immune system.

See full target information CRLF1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 15:9550 PubMed39500869

2024

Inhibition of GPX4 enhances CDK4/6 inhibitor and endocrine therapy activity in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

M T Herrera-Abreu,J Guan,U Khalid,J Ning,M R Costa,J Chan,Q Li,J-P Fortin,W R Wong,P Perampalam,A Biton,W Sandoval,J Vijay,M Hafner,R Cutts,G Wilson,J Frankum,T I Roumeliotis,J Alexander,O Hickman,R Brough,S Haider,J Choudhary,C J Lord,A Swain,C Metcalfe,N C Turner

Journal of translational medicine 19:429 PubMed34656128

2021

miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Li,Jing Xun,Botao Wang,Yuan Ma,Lanqiu Zhang,Lei Yang,Ruifang Gao,Jun Guan,Tianyu Liu,Hejun Gao,Ximo Wang,Qi Zhang

Frontiers in cell and developmental biology 8:858 PubMed33072735

2020

CRLF1 Is a Key Regulator in the Ligamentum Flavum Hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Zheng,Xiang Ao,Peng Li,Zhengnan Lian,Tao Jiang,Zhongmin Zhang,Liang Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CRLF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRLF1 antibody (AB211438)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRLF1 antibody (AB211438)

Western blot - Anti-CRLF1 antibody (AB211438)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise