AB200663

Anti-CSDE1/NRU antibody [EPR17413]

RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CSDE1/NRU antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

대체 명칭 보기

D1S155E, KIAA0885, NRU, UNR, CSDE1, Cold shock domain-containing protein E1, N-ras upstream gene protein, Protein UNR

7 이미지

Supplier Data

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/1000 dilution (Panel A) or ab201688 at 1/1000 dilution (Panel B). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input). Lane 2 : ab200663 IP in HeLa whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 min (Panel A); 10 seconds (Panel B).

All lanes:

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

Predicted band size: 89 kDa

false

Supplier Data

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input). Lane 2 : ab200663 IP in HeLa whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

Predicted band size: 89 kDa,93 kDa

false

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/2000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution

All lanes:

MCF-7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Blocking/Dilution buffer : 5% NFDM/TBST.

The HeLa cell fractions lysate set is ab168542 from Abcam.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/2000 dilution

Lane 1:

HeLa cytosolic fraction at 10 µg

Lane 2:

HeLa membrane (mitochondrial) fraction at 10 µg

Lane 3:

HeLa nuclear fraction at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution

All lanes:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 1min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17413

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/140", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

RNA-binding protein involved in translationally coupled mRNA turnover (PubMed : 11051545, PubMed : 15314026). Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed : 11051545, PubMed : 15314026). Required for efficient formation of stress granules (PubMed : 29395067).. (Microbial infection) Required for internal initiation of translation of human rhinovirus RNA.

See full target information CSDE1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 298:102585 PubMed36223838

2022

Tick-borne encephalitis virus capsid protein induces translational shutoff as revealed by its structural-biological analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Selinger,Radim Novotný,Jakub Sýs,Justin A Roby,Hana Tykalová,Ganji Sri Ranjani,Marie Vancová,Kateřina Jaklová,Filip Kaufman,Marshall E Bloom,Zbyněk Zdráhal,Libor Grubhoffer,Jade K Forwood,Richard Hrabal,Michaela Rumlová,Ján Štěrba

Nucleic acids research 46:11575-11591 PubMed30247708

2018

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Applications

Unspecified application

Species

Unspecified reactive species

Evan P Booy,Ewan Ks McRae,Peyman Ezzati,Taegi Choi,Daniel Gussakovsky,Sean A McKenna

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CSDE1/NRU antibody [EPR17413]

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise