AB228897

Anti-CTF/NFIA antibody

Name: Anti-CTF/NFIA antibody
Brand: Abcam Limited
SKU: AB228897
Rating: 5 (1 reviews)

(1 리뷰)

(12 제품이 사용된 논문 )

Rabbit Polyclonal NFIA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NFIA.

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)

Immunohistochemical analysis of paraffin-embedded human heart tissue labelling NFIA protein with ab228897 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-CTF/NFIA antibody (AB228897)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CTF/NFIA antibody (AB228897)

4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for CTF/NFIA (green) using ab228897 at 1/1000 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labelling NFIA protein with ab228897 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labelling NFIA protein with ab228897 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NFIA. The exact immunogen used to generate this antibody is proprietary information.

Q12857

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The transcription factor CTF/NFIA also known simply as NFIA plays a significant role in gene regulation. It has a molecular weight of approximately 55 kDa. CTF/NFIA is expressed in various tissues with a higher abundance in the central nervous system particularly during development. It binds to specific DNA sequences influencing the transcription of target genes essential for developmental processes and cellular differentiation.

Biological function summary

CTF/NFIA participates in several cellular processes by forming part of a complex that regulates transcription. This complex influences the expression of genes involved in neurodevelopment and gliogenesis. CTF/NFIA acts as a major regulator controlling genes important for the proper formation of brain structure and function. Its ability to modulate gene expression allows it to act as a mediator for cellular environments that require precise genetic control.

Pathways

The protein integrates a network involving the Wnt signaling pathway and the Hedgehog signaling pathway. These pathways are fundamental in maintaining proper cellular growth division and differentiation. In the Wnt signaling pathway CTF/NFIA interacts with the β-catenin complex to control the expression of genes related to cell fate decisions. Similarly within the Hedgehog signaling pathway CTF/NFIA helps in modulating transcriptional responses to developmental cues.

CTF/NFIA has significant implications for brain-related conditions including hydrocephalus and intellectual disabilities. Mutations or dysregulation of NFIA are linked to structural brain malformations that can have severe impacts. Additionally CTF/NFIA interacts with proteins like GLI1 in the context of disease further highlighting its role in signal transduction pathways that when disrupted contribute to these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

See full target information NFIA

대체 명칭 보기

KIAA1439, NFIA, Nuclear factor 1 A-type, NF1-A, Nuclear factor 1/A, CCAAT-box-binding transcription factor, Nuclear factor I/A, TGGCA-binding protein, CTF, NF-I/A, NFI-A

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제품이 사용된 논문 (12)

Recent publications for all applications. Explore the 전체 목록and refine your search

Molecular and clinical oncology 23:72 PubMed40599718

2025

Nuclear factor IA-mediated transcriptional regulation of crystallin αB inhibits hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Jin,Pingping Hu,Yihe Dai,Wenchao Gu,Jiang Han,Haihan Song

eLife 13: PubMed40085500

2025

Dual role of FOXG1 in regulating gliogenesis in the developing neocortex via the FGF signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mahima Bose,Ishita Talwar,Varun Suresh,Urvi Mishra,Shiona Biswas,Anuradha Yadav,Shital T Suryavanshi,Simon Hippenmeyer,Shubha Tole

Neurochemical research 50:77 PubMed39789409

2025

Identification of a Subpopulation of Astrocyte Progenitor Cells in the Neonatal Subventricular Zone: Evidence that Migration is Regulated by Glutamate Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zila Martinez-Lozada,Alain M Guillem,Isabella Song,Michael V Gonzalez,Hajime Takano,Esha Parikh,Jeffrey D Rothstein,Mary E Putt,Michael B Robinson

Nature cell biology 27:347-359 PubMed39779941

2025

Mapping the developmental trajectory of human astrocytes reveals divergence in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin Sojka,Hsiao-Lin V Wang,Tarun N Bhatia,Yangping Li,Pankaj Chopra,Anson Sing,Anna Voss,Alexia King,Feng Wang,Kevin Joseph,Vidhya M Ravi,Jeffrey Olson,Kimberly Hoang,Edjah Nduom,Victor G Corces,Bing Yao,Steven A Sloan

Journal of neuroinflammation 20:247 PubMed37880726

2023

Cell-specific NFIA upregulation promotes epileptogenesis by TRPV4-mediated astrocyte reactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Kong,Tao-Xiang Chen,Xiang-Lei Jia,Xue-Lei Cheng,Meng-Liu Zeng,Jing-Yi Liang,Xiao-Hua He,Jun Yin,Song Han,Wan-Hong Liu,Yuan-Teng Fan,Ting Zhou,Yu-Min Liu,Bi-Wen Peng

iScience 26:107887 PubMed37771660

2023

Acquisition of neural fate by combination of BMP blockade and chromatin modification.

Applications

Unspecified application

Species

Unspecified reactive species

Agnes Lee Chen Ong,Toshiya Kokaji,Arisa Kishi,Yoshihiro Takihara,Takuma Shinozuka,Ren Shimamoto,Ayako Isotani,Manabu Shirai,Noriaki Sasai

Nature communications 14:3372 PubMed37291151

2023

Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Molina-Gonzalez,Rebecca K Holloway,Zoeb Jiwaji,Owen Dando,Sarah A Kent,Katie Emelianova,Amy F Lloyd,Lindsey H Forbes,Ayisha Mahmood,Thomas Skripuletz,Viktoria Gudi,James A Febery,Jeffrey A Johnson,Jill H Fowler,Tanja Kuhlmann,Anna Williams,Siddharthan Chandran,Martin Stangel,Andrew J M Howden,Giles E Hardingham,Veronique E Miron

Frontiers in cardiovascular medicine 9:1062695 PubMed36568562

2022

Large-conductance Ca-activated K channel β1-subunit maintains the contractile phenotype of vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meili Wang,Shuanglei Li,Hongshan Liu,Mingyuan Liu,Jin Zhang,Yang Wu,Cangsong Xiao,Haixia Huang

Development (Cambridge, England) 149: PubMed36196585

2022

Dentate gyrus morphogenesis is regulated by β-catenin function in hem-derived fimbrial glia.

Applications

Unspecified application

Species

Unspecified reactive species

Arpan Parichha,Debarpita Datta,Varun Suresh,Mallika Chatterjee,Michael J Holtzman,Shubha Tole

Mediators of inflammation 2022:5400592 PubMed36254157

2022

miR-181d-5p Protects against Retinal Ganglion Cell Death after Blunt Ocular Injury by Regulating NFIA-Medicated Astrocyte Development.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghua Li,Junyi Liu,Yuanping Zhang,Xu Zha,Hong Zhang,Yongying Tang,Xueying Zhao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CTF/NFIA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)

Immunocytochemistry/ Immunofluorescence - Anti-CTF/NFIA antibody (AB228897)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (12)

Product promise